SMAD3 antibody LS-C18974 is an unconjugated rabbit polyclonal antibody to SMAD3 from human, mouse, rat and other species. Validated for ELISA, IHC and WB.
Human, Mouse, Rat, Bovine, Pig, Chicken, Xenopus, Zebrafish
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:500 - 1:3000)
- Western blot (1:500 - 1:3000)
- ELISA (1:10000 - 1:40000)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
SMAD3 antibody was raised against synthetic peptide from human Smad3.
Amino acids 417-425 of human SMAD3 protein.
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kD in size corresponding to Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. This reagent reacts equally to phosphorylated and nonphosphorylated Smad3 by ELISA and western blotting.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
For research use only.