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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SMAD3 Antibody (aa417‑425) LS‑C18974

Anti-Smad3 Antibody - Immunohistochemistry. Affinity Purified anti-Smad3 antibody shows strong cytoplasmic and membranous staining of tumor cells in cancerous human liver tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Western, www. proteinatlas. org, Uppsala, Sweden.
Anti-Smad3 Antibody - Western Blot. Western blot of affinity purified anti-Smad3 antibody shows detection of endogenous Smad3 in both unstimulated and stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF-beta lanes for 1 hour. This reagent recognizes both non-phosphorylated and phosphorylated Smad3 protein. Personal Communication. Ying Zhang, NIH, CCR, Bethesda, MD.
Anti-Smad3 Antibody - Immunohistochemistry. Affinity Purified anti-Smad3 antibody shows strong cytoplasmic and membranous staining of tumor cells in cancerous human liver tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Western, www. proteinatlas. org, Uppsala, Sweden.
Anti-Smad3 Antibody - Western Blot. Western blot of affinity purified anti-Smad3 antibody shows detection of endogenous Smad3 in both unstimulated and stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF-beta lanes for 1 hour. This reagent recognizes both non-phosphorylated and phosphorylated Smad3 protein. Personal Communication. Ying Zhang, NIH, CCR, Bethesda, MD.
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SMAD3 Antibody (aa417‑425) LS‑C18974

Antibody:
Rabbit Polyclonal to Human SMAD3
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Chicken, Xenopus, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C18974-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human SMAD3
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Chicken, Xenopus, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD3 antibody LS-C18974 is an unconjugated rabbit polyclonal antibody to SMAD3 from human, mouse, rat and other species. Validated for ELISA, IHC and WB.
Target
Human SMAD3
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Pig, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:500 - 1:3000)
  • Western blot (1:500 - 1:3000)
  • ELISA (1:10000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SMAD3 antibody was raised against synthetic peptide from human Smad3.
Epitope
aa417-425
Specificity
Amino acids 417-425 of human SMAD3 protein.
Usage
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kD in size corresponding to Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. This reagent reacts equally to phosphorylated and nonphosphorylated Smad3 by ELISA and western blotting.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD3
P84022 NM_005902 NP_005893.1

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Requested From: United States
Date Requested: 7/23/2019
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