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Catalog Number Size Price
LS-C100546-400 400 µl $393 
SMAD2 Antibody - Fluorescent confocal image of SY5Y cells stained with SMAD2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated SMAD2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the SMAD2 mainly to the nucleus.
SMAD2 Antibody - Western blot of SMAD2 Antibody in NIH-3T3 cell line lysates (35 ug/lane). SMAD2 (arrow) was detected using the purified antibody.
SMAD2 Antibody - SMAD2 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SMAD2 Antibody - Fluorescent confocal image of SY5Y cells stained with SMAD2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated SMAD2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the SMAD2 mainly to the nucleus.
SMAD2 Antibody - Western blot of SMAD2 Antibody in NIH-3T3 cell line lysates (35 ug/lane). SMAD2 (arrow) was detected using the purified antibody.
SMAD2 Antibody - SMAD2 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Polyclonal Rabbit anti‑Human SMAD2 Antibody (Ser118, IF, WB) LS‑C100546

Polyclonal Rabbit anti‑Human SMAD2 Antibody (Ser118, IF, WB) LS‑C100546

Antibody:
SMAD2 Rabbit anti-Human Polyclonal (Ser118) Antibody
Application:
IF, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C100546-400
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SMAD2 Rabbit anti-Human Polyclonal (Ser118) Antibody
Application:
IF, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD2 antibody LS-C100546 is an unconjugated rabbit polyclonal antibody to SMAD2 (Ser118) from human. It is reactive with human and mouse. Validated for Flow, IF and WB.
Target
Human SMAD2
Synonyms
SMAD2 | HMAD-2 | HSMAD2 | JV18-1 | Mad protein homolog smad2 | MADR2 | MADH2 | SMAD 2 | MAD homolog 2 | Mad-related protein 2 | JV18 | Mother against DPP homolog 2 | Mothers against DPP homolog 2 | Sma- and Mad-related protein 2 | SMAD family member 2
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat, Pig, Chicken, Zebrafish, Drosophila, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
Ser118
Specificity
This SMAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-125 amino acids from human SMAD2.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD2
Q15796 NM_005901 NP_005892.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024