Target
Human SMAD2
Synonyms
SMAD2 | HMAD-2 | HSMAD2 | JV18-1 | Mad protein homolog smad2 | MADR2 | MADH2 | SMAD 2 | MAD homolog 2 | Mad-related protein 2 | JV18 | Mother against DPP homolog 2 | Mothers against DPP homolog 2 | Sma- and Mad-related protein 2 | SMAD family member 2
Reactivity
Human
(tested or 100% immunogen sequence identity)
Predicted
Orangutan, Mouse, Rat, Bovine (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
SMAD2 pS465 pS467 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal of human SMAD2 protein.
Specificity
Anti-SMAD2 pS465 pS467 antibody is directed against the phosphorylated form of human Smad2 protein at the pS465 and pS467 residues. Reactivity occurs against human SMAD2. Reactivity with non-phosphorylated human Smad2 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross-reactivity with Smad2 protein from human, mouse, rat, orangutan, and bovine based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Applications
- Western blot (1:1000 - 1:3000)
- ELISA (1:10000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at 4°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee