Human SMAD2

Rabbit

Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Xenopus (tested or 100% immunogen sequence identity)

Zebrafish (at least 90% immunogen sequence identity)

Polyclonal

Unconjugated

Protein G purified

Unmodified

Amino acids 234-249 (DQQLNQSMDTGSPAEL) of human SMAD2 were used as immunogen. Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Rabbit, Horse, Opossum, Chicken, Platypus, Lizard, Xenopus (100%); Panda, Stickleback, Pufferfish, Zebrafish (94%).

aa234-249

The SMAD2 amino acid sequence used as immunogen is 100% homologous in human (isoform 1:467 amino acids; isoform 2:437 amino acids), mouse, rat, horse, cow, chicken and Xenopus.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (2 - 4 µg/ml)

Immunohistochemistry: LS-B2063 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2063 was determined to be 5 ug/ml.

PBS, 0.05% Sodium Azide

Store at 4°C. Do not freeze.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q15796 NCBI: NM_005901 NP_005892.1