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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C389195-100 100 µg $435 
SMAD1+2+3+5 Antibody - Western blot testing of SMAD 1/2/3/5 antibody and rat samples: 1. heart, 2. skeletal muscle, 3. kidney, 4. brain, and human samples: 5. MM453, 6. MM231, 7. HeLa, 8. SMMC-7721, 9. SW620

SMAD1+2+3+5 Antibody (aa442‑456) LS‑C389195

SMAD1+2+3+5 Antibody (aa442‑456) LS‑C389195

Antibody:
Rabbit Polyclonal to Human SMAD1+2+3+5
Application:
WB
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$435 
LS-C389195-100
100 µg
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human SMAD1+2+3+5
Application:
WB
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD1+2+3+5 antibody LS-C389195 is an unconjugated rabbit polyclonal antibody to SMAD1+2+3+5 from human, mouse, rat and other species. Validated for WB.
Target
Human SMAD1+2+3+5
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 1 µg/ml)
Immunogen
Synthetic peptide from the C-terminus of human SMAD1/2/3/5 (GPLQWLDKVLTQMGS, aa442-456 of UniProt Q15797). Percent identity by BLAST analysis: Human, Mouse, Ferret, Sheep, Cat, Bovine, Hamster, Elephant, Rabbit, Horse, Pig, Turkey, Chicken, Platypus, Lizard, Xenopus, Trout, Pufferfish, Zebrafish, Stickleback, Sea squirt, Trichina worm, Dog (100%).
Epitope
aa442-456
Specificity
Human SMAD1+2+3+5
Usage
SMADs are proteins that modulate the activity of transforming growth factor beta ligands. The SMADs, often in complex with other SMADs/CoSMAD, act as transcription factors that regulate the expression of certain genes. Zhu, H et al concluded that targeted ubiquitination of SMADs may serve to control both embryonic development and a wide variety of cellular responses to TGF-beta signals. R-Smads or receptor regulated Smads are a class of proteins that include SMAD1, SMAD2, SMAD3, SMAD5, and SMAD8. In response to signals by the TGF-beta superfamily of ligands these proteins associate with receptor kinases and are phosphorylated at an SSXS motif at their extreme C-terminus. These proteins then typically bind to the common mediator Smad or co-SMAD SMAD4.
Presentation
Lyophilized from PBS, 2.5% BSA, 0.025% sodium azide/thimerosal.
Reconstitution
Reconstitute in sterile distilled water.
Storage
Prior to reconstitution, 4°C. Following reconstitution store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 10/19/2019