Target
Human SLPI / Antileukoproteinase
Synonyms
SLPI | ALK1 | ALP | Antileukoprotease | BLPI | HUSI | Mucus proteinase inhibitor | HUSI-I | SLP1 | Antileukoproteinase | Seminal proteinase inhibitor | WFDC4 | HUSI-1 | Protease inhibitor WAP4 | WAP4
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
Biotin,
Biotin,
Biotin.
Purification
Immunoaffinity purified
Immunogen
Purified, E. coli-derived, recombinant human secretory leukocyte protease inhibitor (SLPI).
Specificity
Recognizes human SLPI in direct ELISA and Western Blot. ~20% cross-reactivity with mSLPI (ELISA). Antibody neutralizes SLPI biological activity.
Applications
- Western blot (0.1 - 0.2 µg/ml)
- ELISA (0.5 - 1 µg/ml)
- Neutralization
Usage
Suitable for use in Neutralization, Direct ELISA, Western Blot. Neutralization: ~20 ng of antibody will neutralize 50% of the activity due to 1 ng of rhSLPI. Inhibits trypsin-catalyzed hydrolysis of tosyl-Gly-Pro-Lys-4-nitroanilide acetate. Recombinant human SLPI will inhibit trypsin activity at a 1:1 molar ratio of rhSLPI to active trypsin. Direct ELISA: 0.5-1.0 ug/ml. The detection limit for rhSLPI is approximately 0.03 ng/well. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human SLPI. The detection limit for rhSLPI is ~1 ng/lane under non-reducing and reducing conditions.
Presentation
PBS, pH 7.2, 40% Glycerol
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SLPI / Antileukoproteinase