SLIT2 | SLIL3 | Slit (Drosophila) homolog 2 | Slit homolog 2 (Drosophila) | Slit homolog 2 protein | Slit-2
(tested or 100% immunogen sequence identity)
Chicken (at least 90% immunogen sequence identity)
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 475-500 of Human SLIT-2 protein.
A BLAST analysis was used to suggest reactivity with this protein from human and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-2 from Tetraodon nigroviridis , as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST). The core 13 amino acids of the immunogen are also 100% homologous to SLIT-2 from mouse, rat, bovine and Xenopus laevis. Cross reactivity with SLIT-2 homologues from other sources has not been determined.
- IHC - Paraffin
- Western blot (1:500 - 1:2000)
- ELISA (1:5000 - 1:50000)
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This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~165 kD corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. For western blotting block the membrane with goat serum. Do not block with non-fat dry milk.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.