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Catalog Number Size Price
LS-C19086-100 100 µg (1 mg/ml) $498 
SLIT2 Antibody - Immunohistochemistry of Rabbit anti SLIT-2 Antibody in human breast carcinoma. Tissue: Human prostate. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker, EDTA pH 9.5 Pressure Cooker. Primary antibody: SLIT2 at 2ug/ml for 1.5 hour @ room Temp. Secondary Ab: MACH 1 HRP POLYMER at 1:50 for 45” RT.
SLIT2 Antibody - Anti-SLIT-2 Antibody - Western Blot. Western blot of Affinity Purified anti-SLIT-2 antibody shows detection of a band at ~165 kD (lane 1) corresponding to SLIT-2 present in a chicken spinal cord whole cell lysate (arrowhead). Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1350. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
SLIT2 Antibody - Immunohistochemistry of Rabbit anti SLIT-2 Antibody in human breast carcinoma. Tissue: Human prostate. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker, EDTA pH 9.5 Pressure Cooker. Primary antibody: SLIT2 at 2ug/ml for 1.5 hour @ room Temp. Secondary Ab: MACH 1 HRP POLYMER at 1:50 for 45” RT.
SLIT2 Antibody - Anti-SLIT-2 Antibody - Western Blot. Western blot of Affinity Purified anti-SLIT-2 antibody shows detection of a band at ~165 kD (lane 1) corresponding to SLIT-2 present in a chicken spinal cord whole cell lysate (arrowhead). Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1350. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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Polyclonal Rabbit anti‑Human SLIT2 Antibody (IHC, WB) LS‑C19086

Polyclonal Rabbit anti‑Human SLIT2 Antibody (IHC, WB) LS‑C19086

Note: This antibody replaces LS-C89074
Antibody:
SLIT2 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C19086-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SLIT2 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
SLIT2 antibody LS-C19086 is an unconjugated rabbit polyclonal antibody to SLIT2 from human. It is reactive with human and chicken. Validated for ELISA, IHC and WB. Cited in 1 publication.
Target
Human SLIT2
Synonyms
SLIT2 | SLIL3 | Slit (Drosophila) homolog 2 | Slit homolog 2 (Drosophila) | Slit homolog 2 protein | Slit-2
Host
Rabbit
Reactivity
Human, Chicken (tested or 100% immunogen sequence identity)
Predicted
Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 475-500 of Human SLIT-2 protein.
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-2 from Tetraodon nigroviridis , as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST). The core 13 amino acids of the immunogen are also 100% homologous to SLIT-2 from mouse, rat, bovine and Xenopus laevis. Cross reactivity with SLIT-2 homologues from other sources has not been determined.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~165 kD corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. For western blotting block the membrane with goat serum. Do not block with non-fat dry milk.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SLIT2
O94813 NM_004787 NP_004778.1

LSBio Ratings

SLIT2 Antibody for IHC, WB/Western, ELISA LS-C19086 has an LSBio Rating of
Publications (4)

Publications (1)

Slit2 overexpression results in increased microvessel density and lesion size in mice with induced endometriosis. Guo SW, Zheng Y, Lu Y, Liu X, Geng JG. Reproductive sciences (Thousand Oaks, Calif.). 2013 Mar;20:285-98.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/14/2022