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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SLIT2 Antibody (aa1416‑1528) IHC‑plus™ LS‑B13918
Note: This antibody replaces LS-C296466
SLIT2 antibody LS-B13918 is an unconjugated rabbit polyclonal antibody to human SLIT2. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B13918-100
100 µl (0.5 mg/ml)
$425
Description
SLIT2 antibody LS-B13918 is an unconjugated rabbit polyclonal antibody to human SLIT2. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SLIT2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, Cy3, HRP, APC, PE, APC, Cy7.
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (15 µg/ml)
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SLIT2 antibody was raised against recombinant Slit2 (Cys1416-Val1528) expressed in E. coli.
Epitope
aa1416-1528
Specificity
The antibody is a rabbit polyclonal antibody raised against Slit2. It has been selected for its ability to recognize Slit2 in immunohistochemical staining and western blotting.
Usage
ELISA 0.05-2ug/ml; ICC 5-20ug/ml; IHCP 15 ug/ml; WB 1-5ug/ml;
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months.
Restrictions
For research use only.
About SLIT2
O94813 NM_004787 NP_004778.1

Popular SLIT2 Products

Anti-SLIT-2 Antibody - Western Blot. Western blot of Affinity Purified anti-SLIT-2 antibody shows detection of a band at ~165 kD (lane 1) corresponding to SLIT-2 present in a chicken spinal cord whole cell lysate (arrowhead). Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1350. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Species: Human, Chicken
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Pig
Applications: Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: Western blot, ELISA
Western blot of recombinant SLIT2 / SLIT-2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: IHC, Western blot
Western blot of recombinant SLIT2 / SLIT-2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot

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Images

Immunohistochemistry - Paraffin

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant SLIT2 / SLIT-2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant SLIT2 / SLIT-2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant SLIT2 / SLIT-2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant SLIT2 / SLIT-2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant SLIT2 / SLIT-2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant SLIT2 / SLIT-2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant SLIT2 / SLIT-2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant SLIT2 / SLIT-2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/18/2018
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