Human SLC9A3R1 / NHERF1 / EBP50

Rabbit

Human, Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Recombinant human His-tagged EBP 50 obtained from bacteria. All His-tag specific antibodies have been removed.

Recognizes EBP 50 from human T84 and Calu3 cells as well as recombinant human protein at ~50kD.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1 µg/ml)
• Immunoprecipitation

Immunohistochemistry: LS-B2196 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2196 was determined to be 5 ug/ml.

PBS, 1 mg/ml BSA, 0.05% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O14745 NCBI: NM_004252 NP_004243.1