NHE3 antibody LS-C16758 is an unconjugated rabbit polyclonal antibody to rat NHE3 (SLC9A3). Validated for ELISA and WB.
(tested or 100% immunogen sequence identity)
- Western blot (1 - 10 µg/ml)
- ELISA (1:100000)
SLC9A3 / NHE3 antibody was raised against synthetic peptide consisting of 22aa within the cytoplasmic, c-terminal domain of the rat NHE3 (KLH coupled).
Recognizes rat NHE3. Species sequence Homology: Mouse: 90%; Human: 76%.
Suitable for use ELISA and Western Blot. ELISA: 1:100000. Using 50-100 ng control peptide/well. Western blot: 1-10 ug/ml (ECL). A band of ~90kD has been detected in brush border membrane.
PBS, pH 7.4, 0.05% Sodium Azide, 0.1% BSA
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.