NCX1 antibody LS-C16737 is an unconjugated rabbit polyclonal antibody to rat NCX1 (SLC8A1). Validated for ELISA and WB.
(tested or 100% immunogen sequence identity)
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:50000)
SLC8A1 / NCX1 antibody was raised against a 23 AA Peptide sequence within the cytoplasmic domain 3 of rat NCX1 (1). The peptide was coupled to KLH.
The 23 AA rat control peptide 100% conserved in mouse, feline, 95% in human, rabbit, monkey, bovine, chicken and 73% in frog NCX1. No significant sequence homology is detected with other NCX. The control peptide is recommend for ELISA, dot blot, or for antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of aff pure IgG or 1 ul of antiserum) to demonstrate antibody specificity.
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure antibody using ECL. ELISA:1:10000-1:50000 for neat serum and 0.5-1 ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1 ug/ml.
PBS, pH 7.4, 0.1% BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.