Rat SLC6A11 / GAT-3
Rat, Human, Mouse
(tested or 100% immunogen sequence identity)
- IHC (2 - 10 µg/ml)
- Western blot (1 - 10 µg/ml)
- ELISA (0.5 - 1 µg/ml)
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SLC6A11 / GAT-3 antibody was raised against a 15 AA synthetic peptide near the C-terminus of rat GAT3 (3). The peptide was synthesized with an added cysteine N-terminus and conjugated to KLH.
Recognizes rat GAT3 without significant homology to any other known eukaryotic protein. Species sequence homology: Mouse-100%, human-92%.
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Western Blot: 1-10 ug/ml using Chemiluminescence technique. We recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands. ELISA: 0.5-1 ug/ml. Control peptide can be used to coat plates at 1 ug/ml. Immunohistochemistry: 2-10 ug/ml in paraformaldehyde fixed sections of tissues.
PBS, pH 7.4, 0.05% Sodium Azide, 40% Glycerol, 0.1% BSA
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.