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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SLC5A3 Antibody (C‑Terminus) IHC‑plus™ LS‑A2760
Note: This antibody replaces LS-C89174, LS-C42478
SLC5A3 antibody LS-A2760 is an unconjugated rabbit polyclonal antibody to SLC5A3 from human, bat, bovine and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A2760-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment

Popular SLC5A3 Products

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Product Description

SLC5A3 antibody LS-A2760 is an unconjugated rabbit polyclonal antibody to SLC5A3 from human, bat, bovine and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human SLC5A3
Synonyms
SLC5A3, Na+/myo-inositol cotransporter, SMIT1, SMIT, BCW2 protein
Host
Rabbit
Reactivity
Human, Bat, Bovine, Dog, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Hamster (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SLC5A3 antibody was raised against synthetic 18 amino acid peptide from C-terminus of human SLC5A3. Percent identity with other species by BLAST analysis: Human, Baboon, Monkey, Galago, Bat, Bovine, Dog, Elephant, Panda, Rabbit, Pig (100%); Marmoset, Mouse, Hamster, Armadillo (94%); Shrew (89%); Rat (83%).
Blocking Peptide
LS-E28622 - Liquid - 50 µg 1 mg/ml - $145.00
Immunizing peptide used to generate LS-A2760. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human SLC5A3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A2760 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A2760 was determined to be 20 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About SLC5A3
Prevents intracellular accumulation of high concentrations of myo-inositol (an osmolyte) that result in impairment of cellular function. P53794 NM_006933 AAC41747.2


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Immunohistochemistry

Anti-SLC5A3 antibody LS-A2760 IHC of human spinal cord. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SLC5A3 antibody LS-A2760 IHC of human spinal cord. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-SLC5A3 antibody LS-A2760 IHC of human spinal cord. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SLC5A3 antibody LS-A2760 IHC of human spinal cord. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-SLC5A3 antibody LS-A2760 IHC of human spinal cord. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SLC5A3 antibody LS-A2760 IHC of human spinal cord. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-SLC5A3 antibody LS-A2760 IHC of human spinal cord. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SLC5A3 antibody LS-A2760 IHC of human spinal cord. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: United States
Date Requested: 10/19/2018

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