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SLC2A5 / GLUT5 Antibody (N-Terminus) LS-C15416

Antibody

SLC2A5 / GLUT5 Antibody (N-Terminus) LS-C15416

Antibody:
Rabbit Polyclonal to Human SLC2A5 / GLUT5
Application:
IHC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$755 
LS-C15416-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human SLC2A5 / GLUT5
Application:
IHC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
GLUT5 antibody LS-C15416 is an unconjugated rabbit polyclonal antibody to human GLUT5 (SLC2A5). Validated for ELISA, IF, IHC and WB.

Target
Human SLC2A5 / GLUT5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • Immunofluorescence
  • Western blot (1 - 10 µg/ml)
  • ELISA (0.5 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SLC2A5 / GLUT5 antibody was raised against a cytoplasmic 12 AA peptide sequence near the C-terminus of human glucose/fructose transporter (Glut-5) (1). A cysteine has been added at the NH2 terminus for coupling to KLH. The peptide was coupled to KLH.
Epitope
N-Terminus
Specificity
Human GT52 sequences shows about 61% homology with rat Glut-5 (8/13 AA). For rat Glut-5, we suggest the use of antibodies that are made to a peptide from rat Glut-5. Antibody cross-reactivity in various species is not established. Control peptide, because of its low mol size (<3kD), is not recommended for Western. It should be used in ELISA, Dot Blot and for antibody blocking to confirm specificity of antibodies.
Usage
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Western Blot: 1-10 ug/ml (ECL). Rat glut-5 is ~60kD in rat jejunum membranes that is slightly larger that that reported for human glut-5 (50-55kD). As opposed to human tissues, rat glut-5 mRNA has not been detected in rat testis, adipose tissues and skeletal muscle. In rat kidney, a protein of ~42kD was detected. ELISA: 0.5-1 ug/ml. Control peptide can be used to coat ELISA plates at 1 ug/ml.
Presentation
PBS, pH 7.4, 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SLC2A5 / GLUT5
P22732 NM_003039 NP_003030.1

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Requested From: United States
Date Requested: 7/15/2019
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