GLUT5 antibody LS-C15416 is an unconjugated rabbit polyclonal antibody to human GLUT5 (SLC2A5). Validated for ELISA, IF, IHC and WB.
Human SLC2A5 / GLUT5
(tested or 100% immunogen sequence identity)
- Western blot (1 - 10 µg/ml)
- ELISA (0.5 - 1 µg/ml)
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SLC2A5 / GLUT5 antibody was raised against a cytoplasmic 12 AA peptide sequence near the C-terminus of human glucose/fructose transporter (Glut-5) (1). A cysteine has been added at the NH2 terminus for coupling to KLH. The peptide was coupled to KLH.
Human GT52 sequences shows about 61% homology with rat Glut-5 (8/13 AA). For rat Glut-5, we suggest the use of antibodies that are made to a peptide from rat Glut-5. Antibody cross-reactivity in various species is not established. Control peptide, because of its low mol size (<3kD), is not recommended for Western. It should be used in ELISA, Dot Blot and for antibody blocking to confirm specificity of antibodies.
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Western Blot: 1-10 ug/ml (ECL). Rat glut-5 is ~60kD in rat jejunum membranes that is slightly larger that that reported for human glut-5 (50-55kD). As opposed to human tissues, rat glut-5 mRNA has not been detected in rat testis, adipose tissues and skeletal muscle. In rat kidney, a protein of ~42kD was detected. ELISA: 0.5-1 ug/ml. Control peptide can be used to coat ELISA plates at 1 ug/ml.
PBS, pH 7.4, 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.