EAAT5 antibody LS-C15442 is an unconjugated rabbit polyclonal antibody to human EAAT5 (SLC1A7). Validated for ELISA and WB.
Human SLC1A7 / EAAT5
(tested or 100% immunogen sequence identity)
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:50000)
SLC1A7 / EAAT5 antibody was raised against a 21AA synthetic peptide near the cytoplasmic, C-terminus of human EAAT5 (1) was synthesized, conjugated to KLH.
The human peptide is 71% conserved in salamander. EAAT5 from other species in not yet cloned. Has no significant sequence homology to other glutamate transporters. Antibody cross-reactivity in various species is not established.
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure IgG using Chemiluminescence technique. ELISA: 1:10000-1:50000 for neat serum and 0.5-1 ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1 ug/ml.
PBS, 0.1% BSA
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.