VACHT antibody LS-C18494 is an unconjugated chicken polyclonal antibody to mouse VACHT (SLC18A3). Validated for ELISA and WB.
Mouse SLC18A3 / VACHT
(tested or 100% immunogen sequence identity)
- Western blot
- ELISA (1:10000 - 1:50000)
SLC18A3 / VACHT antibody was raised against twenty (20) AA peptide mapping within the C-terminus of rat/mouse VAT (1). This peptide is predicted to be cytoplasmic. The peptide was coupled to KLH.
The 20-aa mouse/rat sequence shows 75% homology with the human VAT. No significant sequence homology is seen with other neurotransmitter transporter. Actual species cross-reactivity of antibodies in various species has not been studied.
Suitable for use in ELISA, Western Blot. Western Blot (1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure using ECL. ELISA:1:10000-1:50000 for neat serum and 0.5-1 ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1 ug/ml.
Lyophilized from PBS, 0.1% BSA
Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.
For research use only.