Peptide is 78% conserved in rat and 71% in mouse Npt1. No significant sequence homology is observed with other Pi-transporters (Type-II-IV) transporters. Antibody cross-reactivity in various species is not established.
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane. ELISA:1:10000-1:50000 for neat serum and 0.5-1 ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1 ug/ml.
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
This antibody carries the LSBio 100% Guarantee.