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Catalog Number Size Price
LS-C669806-50 50 µg $294 
LS-C669806-100 100 µg $360 
SLC12A2 / NKCC1 Antibody - Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human salivary gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
SLC12A2 / NKCC1 Antibody - Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
SLC12A2 / NKCC1 Antibody - Immunofluorescence staining of A549 cells with SLC12A2 Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
SLC12A2 / NKCC1 Antibody - Western Blot Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissue All lanes: SLC12A2 antibody at 6.8µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 132, 130 kDa Observed band size: 132 kDa
SLC12A2 / NKCC1 Antibody - Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human salivary gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
SLC12A2 / NKCC1 Antibody - Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
SLC12A2 / NKCC1 Antibody - Immunofluorescence staining of A549 cells with SLC12A2 Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
SLC12A2 / NKCC1 Antibody - Western Blot Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissue All lanes: SLC12A2 antibody at 6.8µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 132, 130 kDa Observed band size: 132 kDa
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Polyclonal Rabbit anti‑Human SLC12A2 / NKCC1 Antibody (IHC) LS‑C669806

Polyclonal Rabbit anti‑Human SLC12A2 / NKCC1 Antibody (IHC) LS‑C669806

Antibody:
SLC12A2 / NKCC1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$294
LS-C669806-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SLC12A2 / NKCC1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
NKCC1 antibody LS-C669806 is an unconjugated rabbit polyclonal antibody to human NKCC1 (SLC12A2). Validated for ELISA and IHC.
Target
Human SLC12A2 / NKCC1
Synonyms
SLC12A2 | Basolateral Na-K-Cl symporter | BSC2 | MBSC2 | NKCC1 | Sy-ns | BSC
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant Human Solute carrier family 12 member 2 protein (110-282AA)
Applications
  • IHC
  • IHC - Paraffin
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SLC12A2 / NKCC1
P55011 NM_001046 NP_001037.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024