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SIRT3 / Sirtuin 3 Antibody (aa304‑334) LS‑C164578

Catalog Number Size Price
LS-C164578-200 200 µl $325 
Sirt3 Antibody IHC of formalin-fixed and paraffin-embedded human breast carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Western blot of lysate from mouse heart tissue lysate, using Mouse Sirt3 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot of Sirt3 Antibody in CHO cell line lysates (35 ug/lane). Sirt3 (arrow) was detected using the purified antibody.
Western blot of Sirt3 Antibody in mouse brain tissue lysates (35 ug/lane). Sirt3 (arrow) was detected using the purified antibody.
Sirt3 Antibody IHC of formalin-fixed and paraffin-embedded human breast carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Western blot of lysate from mouse heart tissue lysate, using Mouse Sirt3 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot of Sirt3 Antibody in CHO cell line lysates (35 ug/lane). Sirt3 (arrow) was detected using the purified antibody.
Western blot of Sirt3 Antibody in mouse brain tissue lysates (35 ug/lane). Sirt3 (arrow) was detected using the purified antibody.
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SIRT3 / Sirtuin 3 Antibody (aa304‑334) LS‑C164578

Antibody:
Rabbit Polyclonal to Mouse SIRT3 / Sirtuin 3
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Human, Hamster
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C164578-200
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Mouse SIRT3 / Sirtuin 3
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Human, Hamster
Format:
Unconjugated, Unmodified

Specifications

Description
Sirtuin 3 antibody LS-C164578 is an unconjugated rabbit polyclonal antibody to Sirtuin 3 (SIRT3 ) from human, mouse and hamster. Validated for IHC and WB.
Target
Mouse SIRT3 / Sirtuin 3
Host
Rabbit
Reactivity
Mouse, Human, Hamster (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8147 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C164578. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa304-334
Specificity
This Sirt3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-334 amino acids from the C-terminal region of Mouse Sirt3.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SIRT3 / Sirtuin 3
Q9NTG7 NM_012239 NP_036371.1

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Western blot of various cell extracts using SIRT 3 (Sirtuin 3, Sir2-related Protein Type 3, Sir2-like 3).
Species: Human, Rat
Applications: Western blot, Immunoprecipitation, ELISA
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Species: Mouse
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Anti-SIRT3 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-7.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
SIRT3 / Sirtuin 3 antibody immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Species: Mouse, Rat, Pig
Applications: Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Fetal Thymus Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit
Applications: Western blot
SIRT3 Antibody western blot of MDA-MB453 cell line lysates (35 ug/lane). The SIRT3 antibody detected the SIRT3 protein (arrow).
Species: Human
Applications: Western blot

Requested From: United States
Date Requested: 8/17/2019
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