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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SIRPA / CD172a Antibody LS-C10175

CD172a antibody LS-C10175 is an unconjugated mouse monoclonal antibody to rat CD172a (SIRPA). Validated for Flow and IHC.
Catalog
Size
Price
LS-C10175-100
100 µg
$515
LS-C10175-250
250 µg
$710
LS-C10175-1
1 mg
$1,020
Description
CD172a antibody LS-C10175 is an unconjugated mouse monoclonal antibody to rat CD172a (SIRPA). Validated for Flow and IHC.
Target
Rat SIRPA / CD172a
Host
Mouse
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Flow Cytometry (1:100 - 1:200)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SIRPA / CD172a antibody was raised against rat peritoneal macrophages.
Specificity
Recognizes rat CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. Binds to a different epitope on SIRP than ED9. Does not block the interaction of CD172a-CD47.
Usage
Suitable for use in Flow Cytometry and Immunohistochemistry (frozen and paraffin). Flow Cytometry: 1:100-1:200. Use 10 ul to label 1E6 cells in 100ul. Immunohistochemistry: Requires protein digestion pre-treatment of paraffin sections e. g. trypsin or pronase.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About SIRPA / CD172a
P78324 NM_080792 NP_542970.1

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Immunofluorescent analysis of CD172a staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRPA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRPA.
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Requested From: United States
Date Requested: 4/21/2019
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