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Catalog Number Size Price
LS-C45263-0.25 0.25 mg (1 mg/ml) $450 
Antibody

Monoclonal Mouse anti‑Rat SIRPA / CD172a Antibody (clone ED9, IHC, WB) LS‑C45263

Monoclonal Mouse anti‑Rat SIRPA / CD172a Antibody (clone ED9, IHC, WB) LS‑C45263

Antibody:
SIRPA / CD172a Mouse anti-Rat Monoclonal (ED9) Antibody
Application:
IHC-Fr, WB, IP, Flo
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$450
LS-C45263-0.25
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SIRPA / CD172a Mouse anti-Rat Monoclonal (ED9) Antibody
Application:
IHC-Fr, WB, IP, Flo
Reactivity:
Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CD172a antibody LS-C45263 is an unconjugated mouse monoclonal antibody to rat CD172a (SIRPA). Validated for Flow, IHC, IP and WB.
Target
Rat SIRPA / CD172a
Synonyms
SIRPA | CD172A | CD172a antigen | Inhibitory receptors shps-1 | Inhibitory receptor SHPS-1 | Macrophage fusion receptor | MyD-1 antigen | p84 | SHPS-1 | SHPS1 | SIRP | Sirp-alpha-1 | Sirp-alpha-3 | Sirp-alpha1 | MYD1 | SIRPalpha | SIRPalpha2 | BIT | MFR | MYD-1 | PTPNS1 | SHP substrate 1 | Sirp-alpha-2 | CD172
Host
Mouse
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
ED9
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Spleen cell homogenate.
Specificity
Recognizes rat CD172a also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. ED9 binds to a different epitope on SIRP than MRC OX-41 and has been reported to block the interaction of CD172a - CD47. Removal of Sodium Azide is recommended prior to use in functional assays. XXX recommend the use of EQU003 for this purpose.
Applications
  • IHC - Frozen (1:100 - 1:1000)
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SIRPA / CD172a
P78324 NM_080792 NP_542970.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/15/2022