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Catalog Number Size Price
LS-C87375-100 100 µg (0.5 mg/ml) $503 
SIGLEC8 Antibody - Antibody (0.3 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Polyclonal Goat anti‑Human SIGLEC8 Antibody (aa134‑145, WB) LS‑C87375

Polyclonal Goat anti‑Human SIGLEC8 Antibody (aa134‑145, WB) LS‑C87375

Antibody:
SIGLEC8 Goat anti-Human Polyclonal (aa134-145) Antibody
Application:
WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C87375-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SIGLEC8 Goat anti-Human Polyclonal (aa134-145) Antibody
Application:
WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SIGLEC8 antibody LS-C87375 is an unconjugated goat polyclonal antibody to human SIGLEC8 (aa134-145). Validated for Peptide-ELISA and WB.
Target
Human SIGLEC8
Synonyms
SIGLEC8 | CDw329 | CD329 antigen | SAF-2 | SIGLEC-8 | Sialoadhesin family member 2 | SIGLEC8L | SAF2
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-SYKSQLNYKTKQ, from the internal region of the protein sequence according to NP_055257.2.
Epitope
aa134-145
Specificity
Human SIGLEC8.
Applications
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 45kD band observed in lysates of cell line MOLT4 (calculated MW of 54.0kD according to NP_055257.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nutku at al, Blood. 2003 Jun 15;101(12):5014-20.; PMID: 12609831). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SIGLEC8
Q9NYZ4 NM_014442 NP_055257.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024