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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SIGLEC6 Antibody (C‑Terminus) LS‑C80472
SIGLEC6 antibody LS-C80472 is an unconjugated rabbit polyclonal antibody to human SIGLEC6. Validated for WB.
Catalog
Size
Price
LS-C80472-50
50 µg
$365

Popular SIGLEC6 Products

Antibody
Species: Human
Applications: Western blot, ELISA
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Western Blot analysis of SIGLEC6 expression in transfected 293T cell line by SIGLEC6 monoclonal antibody (M02), clone 2G6.Lane 1: SIGLEC6 transfected lysate(48.3 KDa).Lane 2: Non-transfected lysate.
Species: Human
Applications: Western blot, Immunoprecipitation, ELISA
Immunohistochemistry of paraffin-embedded human placenta using antibody at dilution of 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunohistochemistry of Human colon cancer using SIGLEC6 Polyclonal Antibody at dilution of 1:70.
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

SIGLEC6 antibody LS-C80472 is an unconjugated rabbit polyclonal antibody to human SIGLEC6. Validated for WB.

Specifications

Target
Human SIGLEC6
Synonyms
SIGLEC6, CDW327, CD33L1, CD327, CD327 antigen, CD33L, OB-BP1, OBBP1, Obesity-binding protein 1, SIGLEC-6, CD33 antigen-like 1, CD33L2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
SIGLEC6 antibody was raised against synthetic peptide from C-Terminus of human SIGLEC6 (O43699, NP_001236). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon (92%).
Blocking Peptide
LS-E15434 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80472. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human SIGLEC6
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About SIGLEC6
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. O43699 NM_001245 NP_001236.4


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Images

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/13/2018

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