Target
Mouse SIGLEC1 / CD169 / Sialoadhesin
Synonyms
SIGLEC1 | CD169 | CD169 antigen | SIGLEC-1 | Sialoadhesin | SN | DJ1009E24.1
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Immunogen
Stromal (reticular) elements from spleen.
Specificity
Specific for CD169, also known as sialoadhesin. The antibody reacts with a subpopulation of mature resident tissue macrophages. No reactivity is seen with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells or blood cells. Distinct macrophage subpopulations of lymphoid organs express the antigen. In the spleen, they are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localized in the sinusoids and medullary cords but not within follicular areas or paracortex. In Peyer's patches they are localized in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained by MOMA-1, although antigen expression is weaker than that seen in splenic macrophages. No MOMA-1 positive macrophages were found in the thymus. Reactivity was also negative in following organs tested so far (kidney, brain, skin). In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine.