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Sheep Anti-Human Kappa Light Chain Secondary Antibody LS-C147797

Ordering

Wt. Vol. Conc. Price
- 1000 µl - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Human Kappa Light Chain Antibodies

Goat Anti-Human Kappa Light Chain Secondary Antibody (Fab'2) LS-C59091
Goat Polyclonal (IgG) to Human Kappa Light Chain
Human
ELISA
Unconjugated, Fab'2
Goat Anti-Human Kappa Light Chain Secondary Antibody (FITC) LS-C60539
Goat Polyclonal to Human Kappa Light Chain
Human
Immunofluorescence, Flow Cytometry
FITC Conjugated
Mouse Anti-Human Kappa Light Chain Secondary Antibody (clone HP6053, Azide-free, HRP) LS-C212476
Mouse Monoclonal [clone HP6053] (IgG1,k) to Human Kappa Light Chain
Human
IHC, Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Sheep Polyclonal to Human Kappa Light Chain
Human
Immunodiffusion, Immunoelectrophoresis
Unconjugated

Details

Human Kappa Light Chain
Sheep
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity absorbed
Unmodified

Applications

  • Immunodiffusion
  • Immunoelectrophoresis

Specificity and Use

Human Ig kappa light chains (free)
Human Ig Kappa Light Chain. The reactivity of the antiserum is directed to the hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is only reacting with Bence Jones proteins as well as free light chains of the kappa type. No reaction is obtained with intact polyclonal or monoclonal immunoglobulins. Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
This product is intended for use to detect and identify free light chain type and Bence Jones protein in serum or urine by immunoelectrophoresis or double radial immunodiffusion. It is of great importance as an aid in the diagnosis of B cell malignancies such a multiple myeloma and non-Hodgkin lymphoma, and in monitoring their therapy. In immunoelectrophoresis use /.2 l of sample in a well with a diameter of / mm against 60 l antiserum in a 20 mm long channel. Electrophoresis for / hour, 20 mA. The detection limit of this technique in serum is about 25 mg/l; in the case /00 times concentrated urine is used the detection limit can be brought down to 0.5 mg/l. The fact that in about 40% of the case the Bence Jones protein was only detectable in either serum or urine emphasizes the necessity to investigate both fluids.

Packaging

Lyophilized antiserum
1,000 µl Sterile distilled water 1 ml
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About Human Kappa Light Chain

There are two types of light chain in humans and other mammals, kappa chain, encoded by the immunoglobulin kappa locus on chromosome 2 and lambda chain, encoded by the immunoglobulin lambda locus on chromosome 22. Only one type of light chain is present in a typical antibody, thus the two light chains of an individual antibody are identical.

Requested From: United States
Date Requested: 12/3/2016

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