Human IgG4

Sheep

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity adsorption with other Ig subclasses and serum proteins

Unmodified

Purified homogenous IgG4 is isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Human IgG4, subclass specific.

• Immunodiffusion

The activity of the antiserum is directed to human IgG4 subclass. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify and measure the concentration of IgG4 subclass in serum or other body fluids. Human IgG constitutes about 75% of the total Immunoglobulins in the serum of normal adults. It consists of four subclasses which are distinguished in isotypic heterogeneity of the gamma chains, and in physicochemical, biological and effector properties. The term subclass designates the high degree of homology in amino acid sequences and antigenic structure. In normal adult serum, the approximate percentage composition of IgG with respect to its subclasses is: IgG1:65%, IgG2:23%, IgG3:8% and IgG4:4%. All four subclasses appear in both isotypes of the light chain. Normal serum thus contains eight different molecular forms of IgG, the absolute and relative distribution being genetically controlled and resulting from individual responses to the antigenicity of the environment. Antigenic determinants carrying isotypic subclass specificity can be situated on any of the three structurally very similar sub-regions o the gamma chains, known as domains. In IgG, some antigenic determinants are shared by two or three subclasses, but not by all four. Consequently, antibodies to such determinants will not be strictly subclass-specific. For the identification of human IgG4 in cells and tissues by immunofluorescence or immunocytochemical peroxidase techniques, or in body fluids by ELISA and Western blotting type procedures.

Delipidated, heat-inactivated, lyophilized antiserum. No preservative.

Reconstitute with 1 ml sterile water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.