Description
SGK3 antibody LS-A106914 is an APC-conjugated rabbit polyclonal antibody to human SGK3. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Allophycocyanin.
Also available
Unconjugated or conjugated with
AP,
Biotin,
FITC,
HRP,
PE.
(This antibody is custom conjugated upon order. Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.)
Immunogen
SGK3 antibody was raised against synthetic 20 amino acid peptide from N-terminus of human SGK3. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Horse, Rabbit (95%); Elephant, Panda, Opossum, Turkey, Chicken (90%); Platypus, Lizard, Pufferfish, Zebrafish (85%); Xenopus (80%).
Specificity
Human SGK3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry:
LS-A7230 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for
LS-A7230 was determined to be 5 ug/ml.
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.