Human SGK3

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Allophycocyanin. Also available Unconjugated or conjugated with AP, Biotin, FITC, HRP, PE.  (This antibody is custom conjugated upon order. Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.)

Immunoaffinity purified

Unmodified

• IHC
• IHC - Paraffin
• ELISA
• (applications tested for the base form of this product only)

SGK3 antibody was raised against synthetic 20 amino acid peptide from N-terminus of human SGK3. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Horse, Rabbit (95%); Elephant, Panda, Opossum, Turkey, Chicken (90%); Platypus, Lizard, Pufferfish, Zebrafish (85%); Xenopus (80%).

N-Terminus

Human SGK3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Immunohistochemistry: LS-A7230 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A7230 was determined to be 5 ug/ml.

PBS, 0.1% Sodium Azide

Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

For research use only.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q96BR1 NCBI: NM_013257 NP_037389.4