Target
Human SETD2
Synonyms
SETD2 | HBP231 | HIF-1 | HIP-1 | HSPC069 | KMT3A | Lysine N-methyltransferase 3A | HIF1 | HSET2 | HYPB | p231HBP | SET2 | Huntingtin yeast partner B | KIAA1732 | SET domain containing 2
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity chromatography
Immunogen
Recombinant full length protein of human SETD2
Specificity
Recognizes endogenous levels of SETD2 protein.
Applications
- ICC
- Immunofluorescence (1:50 - 1:200)
- Western blot (1:500 - 1:2000)
- Immunoprecipitation (1:50 - 1:100)
Usage
Western blot analysis of SETD2 expression in mouse brain (A), mouse lung (B) whole cell lysates. Immunofluorescent analysis of SETD2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee