Bovine Serum Proteins

Rabbit

Bovine (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Antiserum

Unmodified

Pooled whole bovine serum and partly purified serum fractions. Freund's complete adjuvant is used in the first step of the immunization procedure.

Bovine serum proteins.

• Immunodiffusion

In immunoelectrophoresis against pooled serum and enriched serum proteins fractions precipitation can be observed of not less than 15 individual proteins components. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony) to identify the serum protein pattern, or the presence or absence of an individual component. To evaluate the purity of an isolated serum protein including immunoglobulins. Since immuno- precipitation depends on a correct antigen/antibody concentration ratio (zone of equivalence) in the gel medium, the protein analysis by immunoelectrophoresis of serum or any other biological fluid or protein fraction should include different proportions of the reactants. It is not possible to obtain an optimal protein pattern in a single analysis. The electroendosmosis effect of different types of agar on proteins with a different net charge can be used to optimize the resolution power of the test system. Agar Nr. 2 contains sufficient positively charged ions to optimize the resolution of the proteins in the beta-gamma regions, while the alpha regions will become more dense. Highly purified agar (Agar Nordic nr. 1) with low electroendosmosis favors the resolution of the proteins in the alpha regions, while the major components in the beta-gamma region can still be identified.

Delipidated, heat-inactivated, lyophilized antiserum, 0.01% sodium azide.

1 ml Distilled water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.