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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SERPINB4 / SCCA1+2 Antibody (aa60-222, Cy3) LS-C697714

SCCA1+2 antibody LS-C697714 is a Cy3-conjugated rabbit polyclonal antibody to SCCA1+2 (SERPINB4) from human and mouse. Validated for WB.
Catalog
Size
Price
LS-C697714-20
20 µl
$235
LS-C697714-100
100 µl
$345
LS-C697714-200
200 µl
$415
LS-C697714-1
1 ml
$965
LS-C697714-10
10 ml
$3,885
Description
SCCA1+2 antibody LS-C697714 is a Cy3-conjugated rabbit polyclonal antibody to SCCA1+2 (SERPINB4) from human and mouse. Validated for WB.
Target
Mouse SERPINB4 / SCCA1+2
Host
Rabbit
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Cy3. Also available Unconjugated or conjugated with Biotin, FITC, FITC, HRP, APC, PE, APC, Cy7.
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
Recombinant SCCA2 (Asn60-Leu222) expressed in E. coli.
Epitope
aa60-222
Specificity
The antibody is a rabbit polyclonal antibody raised against SCCA2. It has been selected for its ability to recognize SCCA2 in immunohistochemical staining and western blotting.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months.
Restrictions
For research use only.
About SERPINB4 / SCCA1+2
P48594 U19576 AAA92602.1

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Immunohistochemistry image at a dilution of 1:600 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Western blot of recombinant SERPINB4 / SCCA-2.
Species: Human
Applications: IHC, ICC, Western blot, Immunoprecipitation

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Images

Western blot

Western Blot; Sample: Human HepG2 cell lysate; Primary Ab: 5µg/ml Rabbit Anti-Mouse SCCA2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Western Blot; Sample: Human HepG2 cell lysate; Primary Ab: 5µg/ml Rabbit Anti-Mouse SCCA2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Western blot

Western Blot; Sample: Recombinant protein.
Western Blot; Sample: Recombinant protein.

Western blot

Western blot of recombinant SERPINB4 / SCCA1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant SERPINB4 / SCCA1. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot; Sample: Human HepG2 cell lysate; Primary Ab: 5µg/ml Rabbit Anti-Mouse SCCA2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Western Blot; Sample: Human HepG2 cell lysate; Primary Ab: 5µg/ml Rabbit Anti-Mouse SCCA2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Western blot

Western Blot; Sample: Recombinant protein.
Western Blot; Sample: Recombinant protein.

Western blot

Western blot of recombinant SERPINB4 / SCCA1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant SERPINB4 / SCCA1. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot; Sample: Human HepG2 cell lysate; Primary Ab: 5µg/ml Rabbit Anti-Mouse SCCA2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Western Blot; Sample: Human HepG2 cell lysate; Primary Ab: 5µg/ml Rabbit Anti-Mouse SCCA2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Western blot

Western Blot; Sample: Recombinant protein.
Western Blot; Sample: Recombinant protein.

Western blot

Western blot of recombinant SERPINB4 / SCCA1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant SERPINB4 / SCCA1. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot; Sample: Human HepG2 cell lysate; Primary Ab: 5µg/ml Rabbit Anti-Mouse SCCA2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Western Blot; Sample: Human HepG2 cell lysate; Primary Ab: 5µg/ml Rabbit Anti-Mouse SCCA2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Western blot

Western Blot; Sample: Recombinant protein.
Western Blot; Sample: Recombinant protein.

Western blot

Western blot of recombinant SERPINB4 / SCCA1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant SERPINB4 / SCCA1. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 4/19/2019
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