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Catalog Number Size Price
LS-C745329-25 25 µl (1.2 mg/ml) $275 
SERPINB2 / PAI-2 Antibody - Immunohistochemistry of rabbit anti-Plasminogen Activator 2 antibody. Tissue: heart. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-Plasminogen Activator 2 at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Plasminogen Activator II as precipitated red signal with hematoxylin purple nuclear counterstain.
SERPINB2 / PAI-2 Antibody - Western blot using the affinity purified anti-PAI-2 antibody shows detection of endogenous PAI-2 in 50 µg of HeLa whole cell lysates. The band at ~42 kDa (arrowhead) corresponds to PAI-2. Faint non-specific bands are also noted at lower and higher molecular weights positions. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:5,000 dilution of HRP- conjugated Gt-a-Rabbit IgG for 45 min at room temperature with ECL used for detection. Molecular weight estimation was made by comparison to prestained MW markers.
SERPINB2 / PAI-2 Antibody - Immunohistochemistry of rabbit anti-Plasminogen Activator 2 antibody. Tissue: heart. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-Plasminogen Activator 2 at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Plasminogen Activator II as precipitated red signal with hematoxylin purple nuclear counterstain.
SERPINB2 / PAI-2 Antibody - Western blot using the affinity purified anti-PAI-2 antibody shows detection of endogenous PAI-2 in 50 µg of HeLa whole cell lysates. The band at ~42 kDa (arrowhead) corresponds to PAI-2. Faint non-specific bands are also noted at lower and higher molecular weights positions. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:5,000 dilution of HRP- conjugated Gt-a-Rabbit IgG for 45 min at room temperature with ECL used for detection. Molecular weight estimation was made by comparison to prestained MW markers.
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Polyclonal Rabbit anti‑Human SERPINB2 / PAI‑2 Antibody (Internal, IHC, WB) LS‑C745329

Polyclonal Rabbit anti‑Human SERPINB2 / PAI‑2 Antibody (Internal, IHC, WB) LS‑C745329

Antibody:
SERPINB2 / PAI-2 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745329-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
SERPINB2 / PAI-2 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
PAI-2 antibody LS-C745329 is an unconjugated rabbit polyclonal antibody to PAI-2 (SERPINB2) (Internal) from human. It is reactive with human and monkey. Validated for ELISA, IHC and WB.
Target
Human SERPINB2 / PAI-2
Synonyms
SERPINB2 | Monocyte Arg-serpin | HsT1201 | PAI | PLANH2 | Serpin B2 | PAI-2 | PAI2
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Predicted
Primate (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal area of human PAI-2 protein.
Epitope
Internal
Specificity
A BLAST analysis was used to suggest cross reactivity with PAI-2 proteins from several primates based on 94% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Applications
  • IHC (10 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SERPINB2 / PAI-2
P05120 NM_002575 NP_002566.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021