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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SERPINB1 Antibody (aa65‑80) IHC‑plus™ LS‑B11271
Note: This antibody replaces LS-C305846
SERPINB1 antibody LS-B11271 is an unconjugated goat polyclonal antibody to human SERPINB1. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B11271-50
50 µg (0.5 mg/ml)
Unavailable

Popular SERPINB1 Products

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SERPINB1 mouse monoclonal antibody.
Species: Human, Rat, Dog
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Anti-SERPINB1 antibody IHC staining of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11272 concentration 5 ug/ml.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
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Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Western blot of SERPINB1 antibody.
Species: Mouse
Applications: IHC, Western blot

Product Description

SERPINB1 antibody LS-B11271 is an unconjugated goat polyclonal antibody to human SERPINB1. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About SERPINB1
SERPINB1 is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants. P30740 NM_030666 NP_109591.1

SERPINB1 Antibody, Anti-elastase Antibody, EI Antibody, LEI Antibody, Leukocyte elastase inhibitor Antibody, Peptidase inhibitor 2 Antibody, PI-2 Antibody, MNEI Antibody, ELANH2 Antibody, M/NEI Antibody, PI2 Antibody, Serpin B1 Antibody


Specifications

Target
Human SERPINB1
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SERPINB1 antibody was raised against synthetic peptide C-EVHSRFQSLNADINKR from an internal region of human SERPINB1 (NP_109591.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Platypus (100%); Ferret, Panda (94%); Bat, Elephant (88%); Monkey, Sheep, Bovine, Horse, Pig (81%); Rat (75%); Mouse (69%).
Epitope
aa65-80
Specificity
Human SERPINB1
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 43kD band observed in lysates of cell line A549 (calculated MW of 42.7kD according to NP_109591.1). Recommended concentration: 1-3 ug/ml.
Presentation
Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-SERPINB1 antibody IHC staining of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11271 concentration 5 ug/ml.
Anti-SERPINB1 antibody IHC staining of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11271 concentration 5 ug/ml.

Western blot

SERPINB1 antibody (1 ug/ml) staining of A549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SERPINB1 antibody (1 ug/ml) staining of A549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-SERPINB1 antibody IHC staining of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11271 concentration 5 ug/ml.
Anti-SERPINB1 antibody IHC staining of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11271 concentration 5 ug/ml.

Western blot

SERPINB1 antibody (1 ug/ml) staining of A549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SERPINB1 antibody (1 ug/ml) staining of A549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-SERPINB1 antibody IHC staining of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11271 concentration 5 ug/ml.
Anti-SERPINB1 antibody IHC staining of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11271 concentration 5 ug/ml.

Western blot

SERPINB1 antibody (1 ug/ml) staining of A549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SERPINB1 antibody (1 ug/ml) staining of A549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-SERPINB1 antibody IHC staining of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11271 concentration 5 ug/ml.
Anti-SERPINB1 antibody IHC staining of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11271 concentration 5 ug/ml.

Western blot

SERPINB1 antibody (1 ug/ml) staining of A549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SERPINB1 antibody (1 ug/ml) staining of A549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 7/18/2018

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