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Catalog Number Size Price
LS-C744708-100 100 µg (1 mg/ml) $552 
SERPINA1 / Alpha 1 Antitrypsin Antibody - Western Blot of Goat-anti-Alpha-1-Anti-Trypsin Antibody. Lane 1: molecular weight. Lane 2: Goat-anti-Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST. Load: 35 µg per lane under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Primary antibody: Alpha 1 Anti-Trypsin antibody at 1:400 for overnight at 4°C. Secondary antibody: DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT in MB-070 30 buffer. Block: 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Predicted/Observed size: 46.7kDa, ~55kDa for Alpha-1-AntiTrypsin. Other band(s): DyLight 488 Donkey anti-Mouse IgG (blue) and DyLight549 Donkey anti-Rabbit IgG (green) secondary antibodies.
SERPINA1 / Alpha 1 Antitrypsin Antibody - Western Blot of Goat Anti-ALPHA 1 ANTI TRYPSIN Antibody Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: ~1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 1.5 hr at RT. Block: MB-070 buffer overnight at 4°C. Predicted/Observed size: 46.7kDa, ~55kDa for Alpha-1-AntiTrypsin. Other band(s): none.
SERPINA1 / Alpha 1 Antitrypsin Antibody - Western Blot of Goat Anti-ALPHA 1 ANTI TRYPSIN Antibody Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: ~1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 1.5 hr at RT. Block: MB-070 buffer overnight at 4°C. Predicted/Observed size: 46.7kDa, ~55kDa for Alpha-1-AntiTrypsin. Other band(s): none.
SERPINA1 / Alpha 1 Antitrypsin Antibody - Western Blot of Goat-anti-Alpha-1-Anti-Trypsin Antibody. Lane 1: molecular weight. Lane 2: Goat-anti-Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST. Load: 35 µg per lane under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Primary antibody: Alpha 1 Anti-Trypsin antibody at 1:400 for overnight at 4°C. Secondary antibody: DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT in MB-070 30 buffer. Block: 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Predicted/Observed size: 46.7kDa, ~55kDa for Alpha-1-AntiTrypsin. Other band(s): DyLight 488 Donkey anti-Mouse IgG (blue) and DyLight549 Donkey anti-Rabbit IgG (green) secondary antibodies.
SERPINA1 / Alpha 1 Antitrypsin Antibody - Western Blot of Goat Anti-ALPHA 1 ANTI TRYPSIN Antibody Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: ~1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 1.5 hr at RT. Block: MB-070 buffer overnight at 4°C. Predicted/Observed size: 46.7kDa, ~55kDa for Alpha-1-AntiTrypsin. Other band(s): none.
SERPINA1 / Alpha 1 Antitrypsin Antibody - Western Blot of Goat Anti-ALPHA 1 ANTI TRYPSIN Antibody Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: ~1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 1.5 hr at RT. Block: MB-070 buffer overnight at 4°C. Predicted/Observed size: 46.7kDa, ~55kDa for Alpha-1-AntiTrypsin. Other band(s): none.
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Polyclonal Goat anti‑Human SERPINA1 / Alpha 1 Antitrypsin Antibody (Lyophilized, HRP, IHC, WB) LS‑C744708

Polyclonal Goat anti‑Human SERPINA1 / Alpha 1 Antitrypsin Antibody (Lyophilized, HRP, IHC, WB) LS‑C744708

Antibody:
SERPINA1 / Alpha 1 Antitrypsin Goat anti-Human Polyclonal (Lyophilized) (HRP) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Horseradish Peroxidase, Lyophilized
Price
Catalog Number
$552
LS-C744708-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SERPINA1 / Alpha 1 Antitrypsin Goat anti-Human Polyclonal (Lyophilized) (HRP) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Horseradish Peroxidase, Lyophilized

Specifications

Description
Alpha 1 Antitrypsin antibody LS-C744708 is an HRP-conjugated goat polyclonal antibody to human Alpha 1 Antitrypsin (SERPINA1). Validated for ELISA, IHC and WB.
Target
Human SERPINA1 / Alpha 1 Antitrypsin
Synonyms
SERPINA1 | A1A | A1AT | AAT | Alpha 1 Antitrypsin | Alpha-1 protease inhibitor | Alpha-1-antiproteinase | Alpha-1-antitrypsin | Alpha1AT | PRO2275 | Alpha-1-antitrypsin null | PI1 | Serpin A1 | Alpha 1 AT
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Alpha-1-Anti-Trypsin Antibody was produced by repeated immunizations with native a1-Anti-Trypsin protein from human plasma.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified a1-Anti-Trypsin [Human Plasma]. Cross reactivity against a1-Anti-Trypsin from other sources is unknown.
Applications
  • IHC (1:200 - 1:1000)
  • Western blot (1:500 - 1:2500)
  • ELISA (1:1000 - 1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SERPINA1 / Alpha 1 Antitrypsin
P01009 K02212 AAB59495.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/18/2024