Human SEMA3F / Semaphorin 3F
(tested or 100% immunogen sequence identity)
- Western blot (0.5 - 1 µg/ml)
Synthetic peptide from human Semaphorin 3F (TPWTQTQAVRFRFSRATDGALRPM) Percent identity by BLAST analysis: Human (92%); Chimpanzee, Gorilla, Orangutan, Gibbon (88%); Monkey (83%).
Recognizes Semaphorin 3F (Sema3F), recombinant and purified protein. By Western blot the antibody recognizes a protein of 90 kD in total extracts from tissue culture cells producing Sema3F. Semaphorin 3F is expressed ubiquitously with higher levels in lung, brain, spleen, muscle and heart
Western blot: 0.5-1.0 ug/ml using ECL. Suggested antibody dilution buffer is TBST (10 mM Tris-HCl, pH 8.0, 150 mM NaCl with 0.05% Tween 20). Suggested incubation is for two hours at room temperature or overnight at 2-8°C. Immunocytochemistry.
0.02 M PB, 0.25 M NaCl, 0.1% Sodium Azide
Store at 4°C, avoid freezing and store undiluted.
For research use only.
This antibody carries the LSBio 100% Guarantee.
About SEMA3F / Semaphorin 3F