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| Catalog Number | Size | Price |
|---|---|---|
| LS-C60772-2 | 2 mg | $333 |
![Mouse IgG Antibody - Anti-MOUSE IgG Antibody Peroxidase Conjugated - Western Blot. Western blot of Anti-Mouse IgG Antibody Peroxidase Conjugated detecting mouse IgG. Mouse IgG (1.0 mg) was separated on a 4-20% gradient gel and then transferred to Whatman Protran BA 85 nitrocellulose (0.45 micron pore size)., and blocked with 5% BLOTTO (B501-0500) o/n at 4C. The membrane was then probed with a 1:2500 dilution of HRP Rb-a-Mouse IgG [H&L] (p/n Anti-MOUSE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated) for 45 min at RT. FemtoMax Super Sensitive Chemiluminescent HRP Substrate (p/n FEMTOMAX-110). Was added and the image was captured using a BioSpectrum Imaging System with a BioChemi 500 -28C cooled 4.0 Megapixel CCD Camera from UVP (www. uvp. com, Upland, CA). Binning: 5X5. Exposure time: 2 min. Aperture open. No emission or excitation filters used. This image was taken for the unconjugated form of this product. Other forms have not been tested.](https://lsbio-7d62.kxcdn.com/image2/rabbit-anti-mouse-igg-secondary-antibody-hrp-ls-c60772/63046_51239.jpg)
Polyclonal Rabbit Anti‑Mouse IgG Secondary Antibody (HRP, IHC, WB) LS‑C60772
Polyclonal Rabbit Anti‑Mouse IgG Secondary Antibody (HRP, IHC, WB) LS‑C60772
Antibody:
Mouse IgG Rabbit anti-Mouse Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Mouse
Format:
Horseradish Peroxidase, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
Mouse IgG Rabbit anti-Mouse Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Mouse
Format:
Horseradish Peroxidase, Unmodified
Specifications
Description
Mouse IgG antibody LS-C60772 is an HRP-conjugated rabbit polyclonal antibody. Validated for ELISA, IHC and WB.
Host
Rabbit
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Mouse IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, mouse IgG and mouse serum.
Applications
- IHC (1:500 - 1:2500)
- Western blot (1:10000 - 1:30000)
- ELISA (1:50000 - 1:300000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Store at 4°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Featured Products
Species:
Mouse
Applications:
IHC, Western blot, ELISA
Species:
Mouse
Applications:
IHC, Western blot, ELISA
Species:
Mouse
Applications:
IHC, Western blot, ELISA
Species:
Mouse
Applications:
IHC, IHC - Frozen, ELISA
Species:
Mouse
Applications:
IHC, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
