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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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S100A9 / MRP14 Antibody (clone S100A9/1011) LS-C390949

Formalin-fixed, paraffin-embedded human tonsil stained with S100A9 antibody.  This image was taken for the unmodified form of this product. Other forms have not been tested.

S100A9 / MRP14 Antibody (clone S100A9/1011) LS-C390949

Antibody:
Mouse Monoclonal to Human S100A9 / MRP14
Application:
IHC, IHC-P, IHC-Fr, IF, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$295 
LS-C390949-20
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human S100A9 / MRP14
Application:
IHC, IHC-P, IHC-Fr, IF, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
MRP14 antibody LS-C390949 is an unconjugated mouse monoclonal antibody to human MRP14 (S100A9). Validated for Flow, IF and IHC.

Target
Human S100A9 / MRP14
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgM,k Monoclonal
Clone
S100A9/1011
Conjugations
Unconjugated
Purification
PEG purified
Modifications
Unmodified. Also available Azide-free.
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen (0.5 - 1 µg/ml)
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
S100A9 / MRP14 antibody was raised against recombinant human protein.
Specificity
Human S100A9 / MRP14
Usage
Recognizes a 14kDa protein, identified as S100A9 (also known as Calgranulin B or MRP14). expressed by granulocytes, monocytes and by tissue macrophages. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This mAb reacts with neutrophils, monocytes and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections. Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues). Optimal dilution of the S100A9 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Presentation
0.01% BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About S100A9 / MRP14
P06702 NM_002965 NP_002956.1

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Requested From: United States
Date Requested: 5/26/2019
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