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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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S100A9 / MRP14 Antibody (clone CPT/1028, Azide‑free) LS‑C390945
MRP14 antibody LS-C390945 is an unconjugated mouse monoclonal antibody to human MRP14 (S100A9). Validated for Flow, IF and IHC.
Catalog
Size
Price
LS-C390945-100
100 µg (1 mg/ml)
$415

Popular S100A9 / MRP14 Products

Antibody
Species: Mouse
Applications: Western blot, ELISA
Anti-S100A9 / MRP14 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
Anti-S100A9 / MRP14 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: Flow Cytometry

Product Description

MRP14 antibody LS-C390945 is an unconjugated mouse monoclonal antibody to human MRP14 (S100A9). Validated for Flow, IF and IHC.

Specifications

Target
Human S100A9 / MRP14
Synonyms
S100A9, 60B8AG, Calgranulin B, CFAG, CAGB, Calgranulin-B, Calprotectin L1H subunit, CGLB, L1Ag, MRP14, NIF, p14, Protein S100-A9, LIAG, MAC387, MRP-14
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgM,k Monoclonal [CPT/1028]
Conjugations
Unconjugated
Purification
PEG purified
Modifications
Azide-free. Also available Unmodified.
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen (0.5 - 1 µg/ml)
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
S100A9 / MRP14 antibody was raised against recombinant human protein.
Specificity
Human S100A9 / MRP14
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes the L1 or Calprotectin molecule, also called S100A8/9 and MRP8/14, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain. Calprotectin comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Calprotectin has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions. This mAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and is important for identifying macrophages in tissue sections. Optimal dilution of the Calprotectin antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Presentation
Sterile Solution PBS
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
About S100A9 / MRP14
S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. P06702 NM_002965 NP_002956.1


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Images

Immunohistochemistry

Formalin-fixed, paraffin-embedded human tonsil stained with Calprotectin antibody (CPT/1028).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with Calprotectin antibody (CPT/1028). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human tonsil stained with Calprotectin antibody (CPT/1028).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with Calprotectin antibody (CPT/1028). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human tonsil stained with Calprotectin antibody (CPT/1028).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with Calprotectin antibody (CPT/1028). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human tonsil stained with Calprotectin antibody (CPT/1028).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human tonsil stained with Calprotectin antibody (CPT/1028). This image was taken for the unmodified form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/15/2018

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