Mouse S100A8 / MRP8

Goat

Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

E. coli-derived, Recombinant, Murine S100A8 Calgranulin A.

Recognizes murine calgranulin A (S100 calcium-binding Protein A8) at ~10kD. In direct ELISAs and western blots, shows less than 5% cross-reactivity with Recombinant, Murine S100A9, Recombinant, Murine S100A10 and Recombinant, Human S100B.

• ICC
• Western blot (0.1 - 0.2 µg/ml)
• ELISA (0.5 - 1 µg/ml)

Suitable for use in Western Blot, Immunocytochemistry and ELISA. Between each step, cells were washed with PBS containing 0.1% BSA. Western Blot: 0.1-0.2 ug/ml. The detection limit is approximately 25 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. Direct ELISA: 0.5-1 ug/ml with the appropriate secondary reagents to detect mouse S100A8. The detection limit for Recombinant, Murine S100A8 is approximately 0.2 ng/well.

PBS, 5% Trehalose, 40% Glycerol

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P05109 NCBI: NM_002964 NP_002955.2