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Catalog Number Size Price
LS-C662563-10 10 µg $318 
LS-C662563-100 100 µg $470 
RUNX1T1 / ETO Antibody - Immunohistochemistry - Anti-RUNX1T1/ETO antibody
RUNX1T1 / ETO Antibody - Immunohistochemistry - Anti-RUNX1T1/ETO antibody
RUNX1T1 / ETO Antibody - IF analysis of RUNX1T1 using anti-RUNX1T1 antibody RUNX1T1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-RUNX1T1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
RUNX1T1 / ETO Antibody - Western blot - Anti-RUNX1T1/ETO antibody
RUNX1T1 / ETO Antibody - Flow Cytometry analysis of U20S cells using anti-RUNX1T1 antibody. Overlay histogram showing U20S cells stained with anti-RUNX1T1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RUNX1T1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
RUNX1T1 / ETO Antibody - Immunohistochemistry - Anti-RUNX1T1/ETO antibody
RUNX1T1 / ETO Antibody - Immunohistochemistry - Anti-RUNX1T1/ETO antibody
RUNX1T1 / ETO Antibody - IF analysis of RUNX1T1 using anti-RUNX1T1 antibody RUNX1T1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-RUNX1T1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
RUNX1T1 / ETO Antibody - Western blot - Anti-RUNX1T1/ETO antibody
RUNX1T1 / ETO Antibody - Flow Cytometry analysis of U20S cells using anti-RUNX1T1 antibody. Overlay histogram showing U20S cells stained with anti-RUNX1T1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RUNX1T1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human RUNX1T1 / ETO Antibody (IHC, WB) LS‑C662563

Polyclonal Rabbit anti‑Human RUNX1T1 / ETO Antibody (IHC, WB) LS‑C662563

Antibody:
RUNX1T1 / ETO Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662563-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
RUNX1T1 / ETO Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ETO antibody LS-C662563 is an unconjugated rabbit polyclonal antibody to human ETO (RUNX1T1). Validated for ELISA, IHC and WB.
Target
Human RUNX1T1 / ETO
Synonyms
RUNX1T1 | AML1T1 | CBFA2T1 | CDR | Eight twenty one protein | Cyclin-D-related protein | ETO | MTG8 | Protein CBFA2T1 | Protein ETO | ZMYND2 | Protein MTG8
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived human RUNX1T1/ETO recombinant protein (Position: T335-D510).
Specificity
Most abundantly expressed in brain. Lower levels in lung, heart, testis and ovary.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RUNX1T1 / ETO
Q06455 NM_004349 NP_004340.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/24/2024