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RRM2B / P53R2 Antibody (N‑Terminus) LS‑C154254

Catalog Number Size Price
LS-C154254-100 100 µg (0.82 mg/ml) $425 
Anti-RRM2B antibody - Western Blot. Western blot of affinity purified anti-RRM2B antibody shows detection of recombinant (lanes 1 and 3) and endogenous protein (lanes 1 to 4) in whole cell extracts from transfected 293T. Lane 1 contains purified recombinant human p53R2. Lane 2 contains 293T cells transfected with control vector. Lane 3 contains 293T transfected with p53R2-myc. Lane 4:293T transfected with ScRNA. Lane 5:293T transfected with p53R2 SiRNA The band at 40.7 kD, indicated by the bottom arrowhead, corresponds to the expected molecular weight of endogenous RRM2B. The band with the middle arrow corresponds to myc-tagged p53R2 at 41.9kD. The highest band at 44.3 kD corresponds to recombinant p53R2. Primary antibody was diluted to 1 ug/mL and incubated overnight at 4C. ECL detection was used.

RRM2B / P53R2 Antibody (N‑Terminus) LS‑C154254

Note: This antibody replaces LS-C91195
Antibody:
Rabbit Polyclonal to Human RRM2B / P53R2
Application:
IHC, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C154254-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human RRM2B / P53R2
Application:
IHC, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
P53R2 antibody LS-C154254 is an unconjugated rabbit polyclonal antibody to human P53R2 (RRM2B ). Validated for ELISA, IF, IHC, IP and WB. Cited in 3 publications.
Target
Human RRM2B / P53R2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • Immunofluorescence
  • Western blot (1 µg/ml)
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Anti-Human RRM2B/p53R2 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of human RRM2B1 protein. A residue of cysteine was added to facilitate coupling.
Epitope
N-Terminus
Specificity
Anti-Human RRM2B/p53R2 is directed against RRM2B and reacts with the RRM2B from human tissues. Based on sequence we expect this antibody to react as well with RRM2B from other species. Based on 100% homology to the immunizing sequence, one may expect reactivity to chimpanzee and orangutan; based on 93% homology, reactivity to macaque, and 92% to pig and Drosophila.
Usage
RRM2B/p53R2 antibody is suitable for IP, IHC, IF, ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kD in size corresponding to RRM2B1 by western blotting in the appropriate cell lysate or extract.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RRM2B / P53R2
Q7LG56 NM_015713 NP_056528.2

Publications (3)

Ribonucleotide reductase small subunit M2B prognoses better survival in colorectal cancer. Liu X, Lai L, Wang X, Xue L, Leora S, Wu J, Hu S, Zhang K, Kuo ML, Zhou L, Zhang H, Wang Y, Wang Y, Zhou B, Nelson RA, Zheng S, Zhang S, Chu P, Yen Y. Cancer research. 2011 71:3202-13.

Ribonucleotide reductase subunit p53R2 regulates mitochondria homeostasis and function in KB and PC-3 cancer cells. Wang X, Liu X, Xue L, Zhang K, Kuo ML, Hu S, Zhou B, Ann D, Zhang S, Yen Y. Biochemical and biophysical research communications. 2011 410:102-7.

RRM2B suppresses activation of the oxidative stress pathway and is up-regulated by p53 during senescence. Kuo ML, Sy AJ, Xue L, Chi M, Lee MT, Yen T, Chiang MI, Chang L, Chu P, Yen Y. Scientific reports. 2012 2:822.

Reviews (0)

Featured Products

WB of RRM2B antibody. Lane 1: Rat Thymus Tissue Lysate. Lane 2: MCF-7 Cell Lysate. Lane 3: A431 Cell Lysate. Lane 4: HELA Cell Lysate..
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus
Applications: Western blot
Anti-RRM2B / p53R2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat
Applications: IHC - Paraffin, Immunofluorescence, Western blot
Reactivity: Mouse
Range: 62.5-4000 pg/ml
Reactivity: Mouse
Range: 0.156-10 ng/ml
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Dog
Applications: IHC, IHC - Paraffin, Western blot

Requested From: United States
Date Requested: 8/24/2019
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