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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C154254-100 100 µg (0.82 mg/ml) $425 
RRM2B / P53R2 Antibody - Anti-RRM2B antibody - Western Blot. Western blot of affinity purified anti-RRM2B antibody shows detection of recombinant (lanes 1 and 3) and endogenous protein (lanes 1 to 4) in whole cell extracts from transfected 293T. Lane 1 contains purified recombinant human p53R2. Lane 2 contains 293T cells transfected with control vector. Lane 3 contains 293T transfected with p53R2-myc. Lane 4:293T transfected with ScRNA. Lane 5:293T transfected with p53R2 SiRNA The band at 40.7 kD, indicated by the bottom arrowhead, corresponds to the expected molecular weight of endogenous RRM2B. The band with the middle arrow corresponds to myc-tagged p53R2 at 41.9kD. The highest band at 44.3 kD corresponds to recombinant p53R2. Primary antibody was diluted to 1 ug/mL and incubated overnight at 4C. ECL detection was used.

Polyclonal Rabbit anti‑Human RRM2B / P53R2 Antibody (N‑Terminus, IHC, IF, WB) LS‑C154254

Polyclonal Rabbit anti‑Human RRM2B / P53R2 Antibody (N‑Terminus, IHC, IF, WB) LS‑C154254

Note: This antibody replaces LS-C91195
Antibody:
Rabbit Polyclonal to Human RRM2B / P53R2
Application:
IHC, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C154254-100
100 µg
0.82 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human RRM2B / P53R2
Application:
IHC, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
P53R2 antibody LS-C154254 is an unconjugated rabbit polyclonal antibody to human P53R2 (RRM2B) (N-Terminus). Validated for ELISA, IF, IHC, IP and WB. Cited in 3 publications.
Target
Human RRM2B / P53R2
Synonyms
RRM2B | MTDPS8A | MTDPS8B | p53R2 | PEOA5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Orangutan, Pig, Drosophila, Macaque (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Applications
  • IHC
  • Immunofluorescence
  • Western blot (1 µg/ml)
  • Immunoprecipitation
  • ELISA (1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Anti-Human RRM2B/p53R2 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of human RRM2B1 protein. A residue of cysteine was added to facilitate coupling.
Epitope
N-Terminus
Specificity
Anti-Human RRM2B/p53R2 is directed against RRM2B and reacts with the RRM2B from human tissues. Based on sequence we expect this antibody to react as well with RRM2B from other species. Based on 100% homology to the immunizing sequence, one may expect reactivity to chimpanzee and orangutan; based on 93% homology, reactivity to macaque, and 92% to pig and Drosophila.
Usage
RRM2B/p53R2 antibody is suitable for IP, IHC, IF, ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kD in size corresponding to RRM2B1 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RRM2B / P53R2
Q7LG56 NM_015713 NP_056528.2

LSBio Ratings

RRM2B / P53R2 Antibody (N-Terminus) for IHC, IF/Immunofluorescence, WB/Western, IP, ELISA LS-C154254 has an LSBio Rating of
Publications (4.2)

Publications (3)

Ribonucleotide reductase small subunit M2B prognoses better survival in colorectal cancer. Liu X, Lai L, Wang X, Xue L, Leora S, Wu J, Hu S, Zhang K, Kuo ML, Zhou L, Zhang H, Wang Y, Wang Y, Zhou B, Nelson RA, Zheng S, Zhang S, Chu P, Yen Y. Cancer research. 2011 71:3202-13.

Ribonucleotide reductase subunit p53R2 regulates mitochondria homeostasis and function in KB and PC-3 cancer cells. Wang X, Liu X, Xue L, Zhang K, Kuo ML, Hu S, Zhou B, Ann D, Zhang S, Yen Y. Biochemical and biophysical research communications. 2011 410:102-7.

RRM2B suppresses activation of the oxidative stress pathway and is up-regulated by p53 during senescence. Kuo ML, Sy AJ, Xue L, Chi M, Lee MT, Yen T, Chiang MI, Chang L, Chu P, Yen Y. Scientific reports. 2012 2:822.

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Requested From: United States
Date Requested: 5/29/2020