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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B90-100 100 µl (1 mg/ml) $425 
RPE65 Antibody - Human Retinal Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE)
RPE65 Antibody - Anti-RPE65 antibody IHC of human brain cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RPE65 Antibody - Human Retinal Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE)
RPE65 Antibody - Anti-RPE65 antibody IHC of human brain cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ RPE65 Antibody Monoclonal Flo,ICC,IHC,WB LS‑B90

IHCPlus™ RPE65 Antibody Monoclonal Flo,ICC,IHC,WB LS‑B90

Note: This antibody replaces LS-C2878
Antibody:
Mouse Monoclonal to Bovine RPE65
Application:
IHC-P, ICC, WB, Flo
Reactivity:
Bovine, Human, Mouse, Rat, Dog, Pig, Chicken
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-B90-100
100 µl
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Bovine RPE65
Application:
IHC-P, ICC, WB, Flo
Reactivity:
Bovine, Human, Mouse, Rat, Dog, Pig, Chicken
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
RPE65 antibody LS-B90 is an unconjugated mouse monoclonal antibody to RPE65 from human, mouse, rat and other species. Validated for Flow, ICC, IHC and WB. Cited in 1 publication.
Target
Bovine RPE65
Host
Mouse
Reactivity
Bovine, Human, Mouse, Rat, Dog, Pig, Chicken (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
401.8B11.3D9
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC - Paraffin (2.5 - 5 µg/ml)
  • ICC
  • Western blot (1:5000 - 1:10000)
  • Flow Cytometry
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Bovine RPE microsomal membrane proteins.
Specificity
RPE65.
Usage
Immunohistochemistry: LS-B90 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B90 was determined to be 2.5 ug/ml.
Presentation
PBS, 0.05% Sodium Azide
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RPE65
Q16518 NM_000329 NP_000320.1

Validation

RPE65 Antibody - Human Retinal Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Retinal Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Retinal Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE)

RPE65 Antibody - Anti-RPE65 antibody IHC of human brain cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-RPE65 antibody IHC of human brain cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-RPE65 antibody IHC of human brain cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Publications (1)

Endogenous regeneration of damaged retinal pigment epithelium following low dose sodium iodate administration: an insight into the role of glial cells in retinal repair. Machali[Character 144]ska A, Kawa MP, Pius-Sadowska E, Rogi[Character 144]ska D, K[Character 142]os P, Baumert B, Wiszniewska B, Machali[Character 144]ski B. Experimental eye research. 2013 Jul;112:68-78.

Customer Reviews (0)


Requested From: United States
Date Requested: 12/5/2019