(tested or 100% immunogen sequence identity)
- IHC (1:1000 - 1:5000)
- Western blot (1:2000 - 1:10000)
- Flow Cytometry
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This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin conjugated IgG. This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.
Suitable for ELISA, western blotting, fluorescent assays, immunoprecipitation, immunodiffusion, conjugation to magnetic particles (beads) and most other immunological methods requiring lot-to-lot consistency, high titer and specificity. Although not specifically tested, this reagent should also be useful for FACS and other fluorescent immunoassays. R-Phycoerythrin (240 kD) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. Reaction with low molecular fragments is typically noted by western blot.
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.