RPE | Ribulose-phosphate 3-epimerase | RPE2-1
(tested or 100% immunogen sequence identity)
- IHC (1:500 - 1:3000)
- Western blot (1:2000 - 1:20000)
- ELISA (1:200000)
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This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, and R-Phycoerythrin conjugated IgG. This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.
Suitable for immunoblotting (western or dot blot), ELISA as well as other immunochemical assays. R-Phycoerythrin (240 kD) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. Reaction with low molecular weight fragments is typically noted by western blot. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitute in 1 mL of deionized water.
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.