ROBO3 | HGPPS | HGPS | RBIG1 | Roundabout homolog 3 | Roundabout-like protein 3 | RIG1
(tested or 100% immunogen sequence identity)
Mouse (at least 90% immunogen sequence identity)
ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- Peptide Enzyme-Linked Immunosorbent Assay
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave bands at approx 100kDa and 80kDa in Mouse fetal Brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 150kDa according to NP_001158239. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
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