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Catalog Number Size Price
LS-C19102-100 100 µl (85 mg/ml) $498 
Antibody

Polyclonal Rabbit anti‑Human RNF7 Antibody (aa102‑113, IHC, WB) LS‑C19102

Polyclonal Rabbit anti‑Human RNF7 Antibody (aa102‑113, IHC, WB) LS‑C19102

Antibody:
RNF7 Rabbit anti-Human Polyclonal (aa102-113) Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C19102-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
RNF7 Rabbit anti-Human Polyclonal (aa102-113) Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
RNF7 antibody LS-C19102 is an unconjugated rabbit polyclonal antibody to human RNF7 (aa102-113). Validated for ELISA, IHC, IP and WB. Cited in 3 publications.
Target
Human RNF7
Synonyms
RNF7 | CKII beta-binding protein 1 | CKBBP1 | RING-box protein 2 | Rbx2 | Regulator of cullins 2 | Ring finger protein 7 | ROC2 | Zinc RING finger protein SAG
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated followed by sterile filtration
Modifications
Unmodified
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 102-113 of Human ROC2 (C-terminal) coupled to KLH.
Epitope
aa102-113
Specificity
Reacts with human, mouse, C.elgans and zebra fish ROC2. Cross reactivity may also occur with ROC2 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and APC11.
Applications
  • IHC
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • ELISA (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This antibody reacts with human ROC2 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins does occur. A 12.6 kD band corresponding to human ROC2 is detected. Most cell lines expressing ROC2 can be used as a positive control. Researchers should determine optimal titers for other applications.
Presentation
Antiserum, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RNF7
Q9UBF6 NM_014245 NP_055060.1

LSBio Ratings

RNF7 Antibody (aa102-113) for IHC, WB/Western, IP, ELISA LS-C19102 has an LSBio Rating of
Publications (4.2)

Publications (3)

Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase. Kamura T, Koepp DM, Conrad MN, Skowyra D, Moreland RJ, Iliopoulos O, Lane WS, Kaelin WG, Elledge SJ, Conaway RC, Harper JW, Conaway JW. Science (New York, N.Y.). 1999 284:657-61.

ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Ohta T, Michel JJ, Schottelius AJ, Xiong Y. Molecular cell. 1999 3:535-41.

Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. Seol JH, Feldman RM, Zachariae W, Shevchenko A, Correll CC, Lyapina S, Chi Y, Galova M, Claypool J, Sandmeyer S, Nasmyth K, Deshaies RJ, Shevchenko A, Deshaies RJ. Genes & development. 1999 13:1614-26.

Customer Reviews (0)


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Requested From: United States
Date Requested: 8/13/2022