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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RNF144A / RNF144 Antibody (aa156‑184) LS‑C162648

Catalog Number Size Price
LS-C162648-400 400 µl $325 
RNF144A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human breast carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
RNF144A Antibody western blot of MDA-MB453 cell line lysates (35 ug/lane). The RNF144A antibody detected the RNF144A protein (arrow).
RNF144A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human breast carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
RNF144A Antibody western blot of MDA-MB453 cell line lysates (35 ug/lane). The RNF144A antibody detected the RNF144A protein (arrow).
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RNF144A / RNF144 Antibody (aa156‑184) LS‑C162648

Antibody:
Rabbit Polyclonal to Human RNF144A / RNF144
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C162648-400
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human RNF144A / RNF144
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
RNF144 antibody LS-C162648 is an unconjugated rabbit polyclonal antibody to human RNF144 (RNF144A ). Validated for IHC and WB. Cited in 1 publication.
Target
Human RNF144A / RNF144
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E3249 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C162648. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa156-184
Specificity
This RNF144A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-184 amino acids from the Central region of human RNF144A.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RNF144A / RNF144
P50876 NM_014746 NP_055561.2

Publications (1)

Epigenetic silencing of RNF144A expression in breast cancer cells through promoter hypermethylation and MBD4. Zhang Y, Yang YL, Zhang FL, Liao XH, Shao ZM, Li DQ. Cancer medicine. 2018 April;7:1317-1325. (Human)

Reviews (0)

Featured Products

Immunohistochemical analysis of RNF144A staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Anti-RNF144A antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western blot of COLO205 cell lysate using RNF144A Antibody
Species: Human, Mouse
Applications: Western blot
Reactivity: Human
Range: 12.35-1000 pg/ml
Product
Species: Mouse, Human
Applications: Western blot, Immunoprecipitation, Flow Cytometry

Requested From: United States
Date Requested: 8/25/2019
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