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RNF10 Antibody (aa731-780) IHC-plus™ LS-B12589
Note: This antibody replaces LS-C80251
RNF10 antibody LS-B12589 is an unconjugated rabbit polyclonal antibody to RNF10 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
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Review This ProductDescription
RNF10 antibody LS-B12589 is an unconjugated rabbit polyclonal antibody to RNF10 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human RNF10
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
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Immunogen
RNF10 antibody was raised against synthetic peptide located between aa731-780 of human RNF10 (Q8N5U6, NP_055683). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Zebrafish (100%); Stickleback (92%); Turkey, Zebra finch, Chicken, Xenopus (85%); Salmon (80%).
Epitope
aa731-780
Specificity
Human RNF10
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About RNF10
Popular RNF10 Products
Species:
Human
Applications:
IHC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish
Applications:
IHC, IHC - Paraffin, Western blot
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Images
Immunohistochemistry - Paraffin
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 8%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry - Paraffin
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 8%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry - Paraffin
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 8%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry - Paraffin
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 8%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From: United States
Date Requested: 2/20/2019
Date Requested: 2/20/2019
