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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RNF10 Antibody (aa731-780) IHC-plus™ LS-B12589

Note: This antibody replaces LS-C80251
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 8%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 8%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RNF10 Antibody (aa731-780) IHC-plus™ LS-B12589

Note: This antibody replaces LS-C80251
Antibody:
Rabbit Polyclonal to Human RNF10
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B12589-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human RNF10
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
RNF10 antibody LS-B12589 is an unconjugated rabbit polyclonal antibody to RNF10 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target
Human RNF10
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
RNF10 antibody was raised against synthetic peptide located between aa731-780 of human RNF10 (Q8N5U6, NP_055683). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Zebrafish (100%); Stickleback (92%); Turkey, Zebra finch, Chicken, Xenopus (85%); Salmon (80%).
Epitope
aa731-780
Specificity
Human RNF10
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RNF10
Q8N5U6 NM_014868 NP_055683.3

Validation

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Requested From: United States
Date Requested: 7/16/2019
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