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RNF10 Antibody (aa731‑780) IHC‑plus™ LS‑B12589
Note: This antibody replaces LS-C80251
RNF10 antibody LS-B12589 is an unconjugated rabbit polyclonal antibody to RNF10 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
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Species:
Human
Applications:
IHC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish
Applications:
IHC, IHC - Paraffin, Western blot
Product Description
RNF10 antibody LS-B12589 is an unconjugated rabbit polyclonal antibody to RNF10 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Specifications
Target
Human RNF10
Synonyms
RNF10, Ring finger protein 10, RIE2, KIAA0262
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
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Immunogen
RNF10 antibody was raised against synthetic peptide located between aa731-780 of human RNF10 (Q8N5U6, NP_055683). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Zebrafish (100%); Stickleback (92%); Turkey, Zebra finch, Chicken, Xenopus (85%); Salmon (80%).
Epitope
aa731-780
Specificity
Human RNF10
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About RNF10
Transcriptional factor involved in the regulation of MAG (Myelin-associated glycoprotein) expression. Acts as a regulator of Schwann cell differentiation and myelination.
Q8N5U6
NM_014868
NP_055683.3
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Images
Immunohistochemistry - Paraffin
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 8%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry - Paraffin
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 8%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry - Paraffin
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 8%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry - Paraffin
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 8%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From: United States
Date Requested: 11/16/2018
Date Requested: 11/16/2018
