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RHEB

Ras homolog enriched in brain

Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.

Gene Name: Ras homolog enriched in brain
Family/Subfamily: Ras GTPase superfamily IPR001806 , not assigned-Ras GTPase superfamily IPR001806
Synonyms: RHEB, RHEB2, GTP-binding protein Rheb, Ras homolog enriched in brain
Target Sequences: NM_005614 NP_005605.1 Q15382

Publications (1)

1
Select nutrients in the ovine uterine lumen. VI. Expression of FK506-binding protein 12-rapamycin complex-associated protein 1 (FRAP1) and regulators and effectors of mTORC1 and mTORC2 complexes in ovine uteri and conceptuses. Gao H, Wu G, Spencer TE, Johnson GA, Bazer FW. Biology of reproduction. 2009 81:87-100. (IHC-P; Sheep) [PubMed:19299312] Related Antibodies: LS-B543.

☰ Filters
Antibodies (20)
Proteins (8)
Over-Expression Lysate (2)
Primary (20)
Recombinant (6)
RHEB (28)
Human (20)
Mouse (12)
Rat (12)
Bat (3)
Bovine (4)
Chicken (3)
Chimpanzee (3)
Dog (3)
Gibbon (3)
Guinea pig (3)
Horse (3)
Monkey (3)
Pig (3)
Rabbit (3)
Sheep (3)
IHC (14)
IHC-P (8)
WB (15)
ELISA (12)
IF (2)
Peptide-ELISA (1)
rabbit (19)
mouse (1)
Product Group
IHCPlus (3)
IgG (16)
IgG1,k (1)
monoclonal mc (1)
polyclonal pc (19)
2C11 (1)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
Unconjugated (14)
aa107-156 (4)
aa104-134 (1)
No (27)
Yes (1)
GST (1)
GST, N-terminus (2)
His,N-terminus (1)
Myc-DDK (Flag) (3)
Human (7)
293T Cells (1)
E. coli (3)
HEK 293 Cells (2)
Insect Cells (1)
Wheat Germ Extract (1)
Purified (2)
Low endotoxin level (3)
RHEB Antibody - Anti-RHEB antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Select
RHEB Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
0.05 mg/$460
RHEB Antibody - Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Select
RHEB Mouse anti-Human Monoclonal (2C11) Antibody
Mouse, Rat, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$460
RHEB Protein - 12.5% SDS-PAGE of human RHEB stained with Coomassie Blue
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Wheat Germ Extract
GST
45.98 kDa
10 µg/$479; 25 µg/$670
RHEB Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
RHEB Rabbit anti-Human Polyclonal (aa107-156) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$485
RHEB Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
RHEB Rabbit anti-Human Polyclonal (aa107-156) (HRP) Antibody
Chicken, Sheep, Bovine, Rabbit, Mouse, Dog, Guinea pig, Pig, Horse, Bat, Rat, Chimpanzee, Gibbon, Human, Monkey
IHC, IHC-P, WB
HRP Conjugated
100 µl/$469
RHEB Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
RHEB Rabbit anti-Human Polyclonal (aa107-156) (Biotin) Antibody
Chicken, Sheep, Bovine, Rabbit, Mouse, Dog, Guinea pig, Pig, Horse, Bat, Rat, Chimpanzee, Gibbon, Human, Monkey
IHC, IHC-P, WB
Biotin Conjugated
100 µl/$469
RHEB Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
RHEB Rabbit anti-Human Polyclonal (aa107-156) (FITC) Antibody
Chicken, Sheep, Bovine, Rabbit, Mouse, Dog, Guinea pig, Pig, Horse, Bat, Rat, Chimpanzee, Gibbon, Human, Monkey
IHC, IHC-P, WB
FITC Conjugated
100 µl/$469
RHEB Antibody - 1:100 staining mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
RHEB Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
RHEB Protein - Purified recombinant protein RHEB was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
20.3 kDa
20 µg/$1,107
RHEB Antibody - Immunohistochemistry of paraffin-embedded Human liver cancer using RHEB Polyclonal Antibody at dilution of 1:40.
Select
RHEB Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
Select
RHEB Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
100 µg/$341
RHEB Antibody - Immunohistochemistry of paraffin-embedded human colon cancer at dilution of 1:100
Select
RHEB Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
50 µg/$294; 100 µg/$360
Select
RHEB Rabbit anti-Human Polyclonal (HRP) Antibody
Human
ELISA
HRP Conjugated
50 µg/$294; 100 µg/$360
Select
RHEB Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ELISA
Biotin Conjugated
50 µg/$294; 100 µg/$360
Select
RHEB Rabbit anti-Human Polyclonal (FITC) Antibody
Human
ELISA
FITC Conjugated
50 µg/$294; 100 µg/$360
RHEB Antibody - RHEB Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.
Select
RHEB Rabbit anti-Human Polyclonal (aa104-134) Antibody
Human
IHC, IHC-P, WB
Unconjugated
400 µl/$393
RHEB Antibody - Western blot of RHEB pAb in extracts from mouse brain tissue and Hela cells.
Select
RHEB Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
50 µl/$356; 100 µl/$461
RHEB Antibody - Western blot analysis of extracts of various cell lines, using RHEB antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Select
RHEB Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
RHEB Antibody - Immunohistochemistry of paraffin-embedded Human ovarian cancer using RHEB Polyclonal Antibody at dilution of 1:30.
Select
RHEB Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
Select
RHEB Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
100 µg/$357
RHEB Antibody - Western blot of Rheb in rat heart cell lysate with Rheb antibody at (A) 2 and (B) 4 ug/ml.
Select
RHEB Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
0.1 mg/$460
RHEB Antibody - Western Blot (WB) analysis of Mouse Kidney Mouse Brain cells using RHEB Polyclonal Antibody diluted at 1:2000.
Select
RHEB Rabbit anti-Human Polyclonal Antibody
Bovine, Rat, Human
ELISA, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
Select
E. coli
GST, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
20.4kD
10 µg/$321; 50 µg/$534; 500 µg/$1,680; 1 mg/$2,353
Select
293T Cells
Myc-DDK (Flag)
20.3 kDa
20 µg/$150
Select
E. coli
GST, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
20.4 kDa
10 µg/$317; 50 µg/$524
Viewing 1-25 of 28 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).