LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
RFXANK Antibody LS‑C3732
RFXANK antibody LS-C3732 is an unconjugated mouse monoclonal antibody to RFXANK from human, mouse, rat and other species. Validated for ELISA, IF, IHC, IP and WB.
Antibody
Catalog
Size
Price
LS-C3732-100
100 µg
$605

Popular RFXANK Products

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-RFXANK mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-RFXANK mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-RFXANK mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Human tonsil using anti-RFXANK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Human tonsil using anti-RFXANK mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

RFXANK antibody LS-C3732 is an unconjugated mouse monoclonal antibody to RFXANK from human, mouse, rat and other species. Validated for ELISA, IF, IHC, IP and WB.

Specifications

Target
Human RFXANK
Synonyms
RFXANK, ANKRA1, BLS, DNA-binding protein RFXANK, F14150_1, RFXB, RFX-B, Regulatory factor X subunit B, RFX-Bdelta4
Host
Mouse
Reactivity
Human, Mouse, Rat, Dog, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Immunofluorescence
  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
RFXANK antibody was raised against synthetic peptide derived from the spectrin-binding domain of the human ankyrin G protein.
Specificity
Specific for s all splice forms of Ankyrin G containing a spectrin-binding domain. Does not cross-react with related ankyrin isoforms. Species cross-reactivity: Human, mouse, rabbit, rat, canine.
Usage
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. ELISA (native): 0.1-1.0 ug/ml. Detects a band between ~200-300kD. Western Blot: 1-3 ug/ml. For best results on rat brain, mouse brain or MDCK cell lysates, add 0.3 M sucrose to incubation buffer. Immunofluorescence (frozen sections): 5-10 ug/ml. Sensitive to fixation conditions. Recommend 1% paraformaldehyde for perfusion and 4 hrs of post fixation, after perfusion. For sciatic nerve, recommend 2% paraformaldehyde for 2 hrs at 4?C. Immunoprecipitation (native): 5 ug/ IP.
Presentation
PBS, pH 7.2, 0.1% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About RFXANK
Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. O14593 NM_003721 NP_003712.1


Publications (0)


Customer Reviews (0)


Requested From: United States
Date Requested: 10/19/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy