RFXANK antibody LS-C3732 is an unconjugated mouse monoclonal antibody to RFXANK from human, mouse, rat and other species. Validated for ELISA, IF, IHC, IP and WB.
Human, Mouse, Rat, Dog, Rabbit
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- IHC - Frozen
- Western blot (1 - 3 µg/ml)
- ELISA (0.1 - 1 µg/ml)
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
RFXANK antibody was raised against synthetic peptide derived from the spectrin-binding domain of the human ankyrin G protein.
Specific for s all splice forms of Ankyrin G containing a spectrin-binding domain. Does not cross-react with related ankyrin isoforms. Species cross-reactivity: Human, mouse, rabbit, rat, canine.
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. ELISA (native): 0.1-1.0 ug/ml. Detects a band between ~200-300kD. Western Blot: 1-3 ug/ml. For best results on rat brain, mouse brain or MDCK cell lysates, add 0.3 M sucrose to incubation buffer. Immunofluorescence (frozen sections): 5-10 ug/ml. Sensitive to fixation conditions. Recommend 1% paraformaldehyde for perfusion and 4 hrs of post fixation, after perfusion. For sciatic nerve, recommend 2% paraformaldehyde for 2 hrs at 4?C. Immunoprecipitation (native): 5 ug/ IP.
PBS, pH 7.2, 0.1% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.