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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RFX5 Antibody (aa320-494) LS-C18752

Note: This antibody replaces LS-C9969
RFX5 antibody LS-C18752 is an unconjugated rabbit polyclonal antibody to human RFX5. Validated for ELISA, IP and WB.
Catalog
Size
Price
LS-C18752-100
100 µg (1 mg/ml)
$425
Description
RFX5 antibody LS-C18752 is an unconjugated rabbit polyclonal antibody to human RFX5. Validated for ELISA, IP and WB.
Target
Human RFX5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • Immunoprecipitation
  • ELISA
Epitope
aa320-494
Specificity
RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Usage
Suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against RFX5 (aa 320 to 494) from a variety of fibroblast and B-cell lysates at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884. Anti- RFX5 (aa 320 to 494) detects a 75 kD band by immunoblot for human RFX5. This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 ul per assay.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About RFX5
P48382 NM_000449 NP_000440.1

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Images

Western blot

Western blot of Rabbit Anti-RFX5 antibody. Lane 1: Raji B cell nuclear extract lysates. Lane 2: none. Load: 35 ug per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 65.3 kDa, 72 kDa for RFX5. Other band(s): none.
Western blot of Rabbit Anti-RFX5 antibody. Lane 1: Raji B cell nuclear extract lysates. Lane 2: none. Load: 35 ug per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 65.3 kDa, 72 kDa for RFX5. Other band(s): none.

Western blot

Western blot of Rabbit Anti-RFX5 antibody. Lane 1: Raji B cell nuclear extract lysates. Lane 2: none. Load: 35 ug per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 65.3 kDa, 72 kDa for RFX5. Other band(s): none.
Western blot of Rabbit Anti-RFX5 antibody. Lane 1: Raji B cell nuclear extract lysates. Lane 2: none. Load: 35 ug per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 65.3 kDa, 72 kDa for RFX5. Other band(s): none.

Western blot

Western blot of Rabbit Anti-RFX5 antibody. Lane 1: Raji B cell nuclear extract lysates. Lane 2: none. Load: 35 ug per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 65.3 kDa, 72 kDa for RFX5. Other band(s): none.
Western blot of Rabbit Anti-RFX5 antibody. Lane 1: Raji B cell nuclear extract lysates. Lane 2: none. Load: 35 ug per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 65.3 kDa, 72 kDa for RFX5. Other band(s): none.

Western blot

Western blot of Rabbit Anti-RFX5 antibody. Lane 1: Raji B cell nuclear extract lysates. Lane 2: none. Load: 35 ug per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 65.3 kDa, 72 kDa for RFX5. Other band(s): none.
Western blot of Rabbit Anti-RFX5 antibody. Lane 1: Raji B cell nuclear extract lysates. Lane 2: none. Load: 35 ug per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 65.3 kDa, 72 kDa for RFX5. Other band(s): none.

Requested From: United States
Date Requested: 4/23/2019
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